5. Answer the following questions.a. Write the name and structure of the product obtained by thereaction of B-D-glucopyranose with acetic anhydride in thepresence of pyridine at 0 °C? (water or ether)b. Which solvent, water or ether, would you prefer to purify thestarting material?c. Which solvent is preferred for the purification andrecrystallization of the final product from the reaction.

Glucose is hexose sugar that forms a pyranose ring structure in solution. It is an aldose sugar…

Answered: 5. Answer the following questions. a.…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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If catalase is tested with the biuret reagent, what would be the ideal result? Why? If catalase is mixed with heavy metals, will it increase its activity of H2O2, yes or no? Defend your answer.
01. Electrophilic aromatic substitution of benzene will not proceed without Lewis acid catalyst. Give the reagent (including catalyst) and the product for the following reactions of benzene. a. nitration b. chlorination c. alkylation d. acylation e. sulfonation 02. Show the Friedel-Crafts acylation reaction of anisole and nitrobenzene with acetic anhydride? Explain the different products formed. 03. What is the basis in the separation of the components of a mixture in chromatography? 04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation. 05. What is the difference between Rf value and retention time (RT)? 06. The chromatogram represents the separation of the components of a mixture after development. Calculate the Rf values of the three components of sample X in the chromatogram. If the SP is silica gel C-18, which is the polar component and the nonpolar component? nchot
A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCl5)) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol. Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2? Please show all calculations and give correct answers.
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl-, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
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II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1)3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Based on the results above, _________________ 1. _________________ 2. _________________ 3. _________________ 4. _________________ 5. answer the following questions: What visible positive result for test reagent no. 5?Give the name of chemical characterization test for test reagent no. 3?What class of lipids will give a visible positive result on the test mentioned in question no. 2?Give the chemical name of the product formed after giving a positive result for test reagent no. 1?Test reagent no. 1 is used to test for what organic compound?
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