N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonicA purified protein is in a Hepesacid) buffer at pH 7 with 375 mM NaCl. Adialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L ofthe same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl-, and Hepes) can todiffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.Calculate the final concentration of NaCl in the proteinsample once the dialysis has come to equilibrium.Calculate the final NaCl concentration in the 2.0 mL proteinsample after dialysis in 150 mL of the same Hepes buffer,with 0 mM NaCl, twice in succession.[NaCl] after a single dialysis:[NaCl] after a double dialysis:mMmM

In this experimental scenario, a purified protein resides in a Hepes buffer at pH 7 with an initial…

Answered: N-(2-hydroxyethyl)piperazine-N'-(2-etha…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Calculate the appropriate volume (in µL) of 3X loading buffer that should be added to 24.0 µL of a sample prior to loading the sample on an agarose gel.
Mike has determined that enzyme he is attempting to purify has an isoelectric point of 4.5 (pI = 4.5). He has decided to examine anion exchange chromatography as a potential purification step. He tested out using 2 different buffer and linear NaCl gradient on HPLC (like what you did). His results are shown below. Which buffer should Mike uses for his purification (both buffer has pH higher than enzyme’s pI)? Why?
Gel Running Buffer is made and kept at 14X concentration for storage. We will need 1.5 L of this solution at a concentration of 1X to run our gels. How would we make this solution? I am having trouble understanding the steps to this question, if you could please help me understand it that would be greatly appreciated!
Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…
a 50-year-old woman with a body mass index in the obese range had a weakness in their muscles. she had a history of increased blood pressure but controlled it with medication. the GP has diagnosed her with extreme hypertension and therefore has given her an increased dosage of blood pressure medication but did not have an effect on her hypertension. the biochemical profile shows: - urea of 8.9 (reference range: 2.5-7.0) - sodium of 162 (reference range: 134-147) - potassium of 2.2 (reference range: 3.5-5.0) -aldosterone of 863 (reference range: 90-700) - renin activity of 4.3 (reference range: 0.5-3.5) - aldosterone/RA ratio of 201 (reference range: <680) -cortisol of 511 (reference range: 138-690) -pH of 7.51 ( reference range : 7.35-7.45) Questions: a)what is the medical condition the patient has and what are the causes? fully explain the rationale which is based on the patients history and the test results indicated above. b)which non invasive test will be taken into…
A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).
Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.
Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose s and adding 50 mL of concentrated solution. Sonya who weighs weighs 60 kg requires a tirofiban dose of 0.4 mcg/kg/min for 30 minute- What volume would remain after 30 minutes? Answer: 94.53 mL X The correct answer is: 236 mL
E. How would you make 8 mL of Wash Buffer (1X Lysis Buffer plus 30 mM Imidazole) from stocks of 2X Lysis buffer and 4 Imidazole?
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.
in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45
)emeul 20g Wq 1h is or dered, o)emerol is supplied in a 25mg Iml Single -use ampule How much o)emerol is wasted with each dose ?

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS