N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonicA purified protein is in a Hepesacid) buffer at pH 7 with 375 mM NaCl. Adialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L ofthe same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl-, and Hepes) can todiffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.Calculate the final concentration of NaCl in the proteinsample once the dialysis has come to equilibrium.Calculate the final NaCl concentration in the 2.0 mL proteinsample after dialysis in 150 mL of the same Hepes buffer,with 0 mM NaCl, twice in succession.[NaCl] after a single dialysis:[NaCl] after a double dialysis:mMmM

In this experimental scenario, a purified protein resides in a Hepes buffer at pH 7 with an initial…

Answered: N-(2-hydroxyethyl)piperazine-N'-(2-etha…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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