03. What is the basis in the separation of the components of a mixture in chromatography? 04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation. 05. What is the difference between Rf value and retention time (RT)?
03. What is the basis in the separation of the components of a mixture in chromatography? 04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation. 05. What is the difference between Rf value and retention time (RT)?
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter3: Biological Macromolecules
Section: Chapter Questions
Problem 6RQ: The following chemical reactants produce the ester ethyl ethanoate (C4H8O2): C2H6O + CH3COOH What...
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![01. Electrophilic aromatic substitution of benzene will not proceed without Lewis acid catalyst. Give
the reagent (including catalyst) and the product for the following reactions of benzene.
a. nitration
b. chlorination
c. alkylation
d. acylation
e. sulfonation
02. Show the Friedel-Crafts acylation reaction of anisole and nitrobenzene with acetic anhydride?
Explain the different products formed.
03. What is the basis in the separation of the components of a mixture in chromatography?
04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation.
05. What is the difference between Rf value and retention time (RT)?
06. The chromatogram represents the separation of the
components of a mixture after development. Calculate the
Rf values of the three components of sample X in the
chromatogram. If the SP is silica gel C-18, which is the polar
component and the nonpolar component?
nchot](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F87c30242-f62f-4562-b9b0-eb4131dfd578%2Fd8f3afaa-de50-4ed3-b4cb-f2cebb2a7dd1%2Fpby9lw_processed.jpeg&w=3840&q=75)
Transcribed Image Text:01. Electrophilic aromatic substitution of benzene will not proceed without Lewis acid catalyst. Give
the reagent (including catalyst) and the product for the following reactions of benzene.
a. nitration
b. chlorination
c. alkylation
d. acylation
e. sulfonation
02. Show the Friedel-Crafts acylation reaction of anisole and nitrobenzene with acetic anhydride?
Explain the different products formed.
03. What is the basis in the separation of the components of a mixture in chromatography?
04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation.
05. What is the difference between Rf value and retention time (RT)?
06. The chromatogram represents the separation of the
components of a mixture after development. Calculate the
Rf values of the three components of sample X in the
chromatogram. If the SP is silica gel C-18, which is the polar
component and the nonpolar component?
nchot
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