1. Why ninhydrin is used in paper chromatography?   2. How do you identify amino acids on the chromatogram?

Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…

A: Serial dilution is a process in which we dilute the sample and do passage, transfer, which is…

Q: (a) You decide to purify the 5-HT 2A receptor. The protein has a predicted molecular Mass of 46.54…

A: Formation of the peptide bond neutralises any charge due carboxyl group, therefore the net charge on…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: What are other chromatographic techniques that can be used for separating non-polar biomolecules…

A: Introduction: Chromatography is a method for separating a mixture in the laboratory. The mixture is…

Q: In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: why steroids do not show luminescence on thin layer chromatographic plates?

A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…

Q: briefly explain the physical mechanism by which amino acids are separated during paper…

A: Paper chromatography is a type of plane chromatography where the stationary phase is present on a…

Q: What is similar and what is different between the following chromatographic methods: Size Exclusion…

A: Chromatography  is a method used for separation of compounds.and they are classified into two…

Q: Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid,…

A: Thin-layer chromatography is a method of separating non-volatile mixtures. Thin-layer chromatography…

Q: In your first paragraph you should explain the theory of chromatography in general, and the behind…

A: Chromatography is a biophysical laboratory technique which is used to separate, identify and allow…

Q: 25. Assume we are performing a 2x serial dilution series of methylene similar to WL-1. If your…

A: The process of making a solution involves dissolving a solute in a solvent to achieve a particular…

Q: Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction…

A: Introduction: Chromatography is a physical method for the separation of compounds. Tswet, a Russian…

Q: In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used…

A: UV/Visible Spectrophotometry detects the absorbance of light in the UV/Visible range of the…

Q: in paper chromatography what is the basis of pigments separate?

A: Paper chromatography is a technique in which different types of pigments can be separated from each…

Q: (b) Table Q1 below provides optical measurements of samples with known concentrations of analyte…

A: When a substance or analyte absorb the light of specific wavelength and emits fluorescence, then the…

Q: o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the…

A: A working solution is different from Stock solution. A Working solution is prepared from stock…

Q: I'm very confused on #13. Can anyone explain if the claim is valid or not? I don't understand how…

A: 13. Purines and pyrimidine nucleotides fill a variety of metabolic roles. they are the "energy…

Q: 1. A.) Which compound acts as dehydrating agent in Molisch's test?  B.) With the correct answer…

A: Disclaimer: Since you have posted a question with multiple sub-parts, we will solve first three…

Q: Why is it important to maintain a constant volume of water in your water bath (when boiling nucleic…

A: Water baths are constant temperature laboratory equipment used to heat samples. The simpleprinciple…

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: Why can't we determine Vo, Ve and Vt for affinity chromatography?

A: Chromatographic techniques separate macromolecules based on size, ion exchange, or their affinity…

Q: 5) Identify the different phases illustrated here. Why are there so many that look like the one in…

A: The cell is the basic unit of life and each living organism is made up of cells. The cell keeps on…

Q: You are analyzing the peptide ala-ile-glu-lys-phe-val- tyr-cys. If you treat the peptide with…

A: Proteins or peptides are a linear chain of amino acids attached together via peptide bonds. Natural…

Q: What is the basic purpose of the paper chromatography?

A: Chromatography is an important analytical method used to separate a mixture of chemical substances…

Q: What is the driving force behind paper chromatography?

A: Chromatography is a laboratory method of separating the mixture into individual components. It…

Q: a) A student performed a thin layer chromatographic separation experiment. In he first run, four…

A: Chromatography is generally a method that helped to separate components in a mixture and also these…

Q: What does an A280 of 0.000 indicate? What does this reading indicate in the context of your column…

A: Meaning: An A280 absorbance of 0.000 at a wavelength of 280 nm suggests an extremely low or…