Homework Help is Here – Start Your Trial Now!
Science
Biology
1.Which of the sample dyes are negatively charged at neutral pH? 2.Which of the sample dyes are positively charged at neutral pH? 3.1: Did Orange G, Xylene Cyanol and Safranin O move the same distance in the gel? 3.2 : Why or why not? What compounds do you suspect are in the unknown sample? Explain.

1.Which of the sample dyes are negatively charged at neutral pH? 2.Which of the sample dyes are positively charged at neutral pH? 3.1: Did Orange G, Xylene Cyanol and Safranin O move the same distance in the gel? 3.2 : Why or why not? What compounds do you suspect are in the unknown sample? Explain.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
100%

1.Which of the sample dyes are negatively charged at neutral pH?

2.Which of the sample dyes are positively charged at neutral pH?

3.1: Did Orange G, Xylene Cyanol and Safranin O move the same distance in the gel?
3.2 : Why or why not? What compounds do you suspect are in the unknown sample? Explain.

**Gel Electrophoresis of Dyes: Per. 1 Sample Results** Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. This method involves the use of a gel matrix and an electric field to separate molecules based on their size and charge. When dyed samples are applied to the gel, they migrate at different rates, allowing for their identification and analysis. In the provided image, the results of a gel electrophoresis experiment of various dyes are shown. Here are the details of the image: 1. **Orientation and Setup:** - Gel electrophoresis apparatus is displayed with the negative pole (-) at the bottom left and the positive pole (+) at the bottom right. 2. **Dye Samples:** - Labeled from top to bottom on the left side of the gel: - Unknown - Xylene Cyanol - Janus Green (replacement) - Bromophenol Blue - Labeled from top to bottom on the right side of the gel: - Unknown - Safranin O - Orange G 3. **Results:** - **Xylene Cyanol:** Visible as a light blue spot near the starting point, indicating it did not travel far and is likely a larger molecule or less negatively charged. - **Janus Green (replacement):** Visible as a bright red spot, which has moved a moderate distance, suggesting an intermediate size and charge. - **Bromophenol Blue:** Visible as a dark blue spot, which has traveled farther than Xylene Cyanol, indicating it is smaller or more negatively charged. - **Unknown (left column):** Appears to have multiple colors, possibly consisting of different components. - **Safranin O:** Marked by a light reddish spot, which indicates it has traveled a moderate distance. - **Orange G:** Displayed as an orange spot, having traveled a small distance, indicative of its size and charge characteristics. - **Unknown (right column):** Consists of multiple colors and patterns, demonstrated similarly to the unknown in the left column. Each dye moved at different rates based on its size and charge, allowing for comparative analysis. This experiment demonstrates how gel electrophoresis can be utilized to analyze and differentiate various chemical compounds based on their electrophoretic mobility.
Transcribed Image Text:**Gel Electrophoresis of Dyes: Per. 1 Sample Results** Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. This method involves the use of a gel matrix and an electric field to separate molecules based on their size and charge. When dyed samples are applied to the gel, they migrate at different rates, allowing for their identification and analysis. In the provided image, the results of a gel electrophoresis experiment of various dyes are shown. Here are the details of the image: 1. **Orientation and Setup:** - Gel electrophoresis apparatus is displayed with the negative pole (-) at the bottom left and the positive pole (+) at the bottom right. 2. **Dye Samples:** - Labeled from top to bottom on the left side of the gel: - Unknown - Xylene Cyanol - Janus Green (replacement) - Bromophenol Blue - Labeled from top to bottom on the right side of the gel: - Unknown - Safranin O - Orange G 3. **Results:** - **Xylene Cyanol:** Visible as a light blue spot near the starting point, indicating it did not travel far and is likely a larger molecule or less negatively charged. - **Janus Green (replacement):** Visible as a bright red spot, which has moved a moderate distance, suggesting an intermediate size and charge. - **Bromophenol Blue:** Visible as a dark blue spot, which has traveled farther than Xylene Cyanol, indicating it is smaller or more negatively charged. - **Unknown (left column):** Appears to have multiple colors, possibly consisting of different components. - **Safranin O:** Marked by a light reddish spot, which indicates it has traveled a moderate distance. - **Orange G:** Displayed as an orange spot, having traveled a small distance, indicative of its size and charge characteristics. - **Unknown (right column):** Consists of multiple colors and patterns, demonstrated similarly to the unknown in the left column. Each dye moved at different rates based on its size and charge, allowing for comparative analysis. This experiment demonstrates how gel electrophoresis can be utilized to analyze and differentiate various chemical compounds based on their electrophoretic mobility.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Microbial isolation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS