In your first paragraph you should explain the theory of chromatography in general, and the behind thin layer chromatography in detail. a) Define the terms "stationary phase" and "mobile phase" and describe how the stationary phase and the mobile phase interact with the analyte during a chromatographic separation (for chromatography in general – the same concept applies to thin layer, column, and gas chromatography). b) For what two main reasons would a chemist perform a thin layer chromatography analysis? c) define and describe the process of thin layer chromatography. Include the following terms: marking, spotting, developing, visualizing, and measuring. Include in your description several different ways that the plates can be visualized.

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: The before and after fluids were observed to detect the movement of salt across the dialyzing…

A: Dialysis is defined as a type of laboratory technique where it removes the salt or reduce the…

Q: The basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of…

A: Radiography is a well-known imaging technique that uses X-rays, gamma rays, or other ionizing and…

Q: You are the RT of the patient, you are asked to suction a newly intubated patient with a 7mm ET.…

A: Suctioning is a very common procedure done for the intubated patients. Suctioning should be done…

Q: Given the GC chromatogram below: 500000 400000 100000 10.767 21.62622 977 13.644 12.343 17938 200000…

A: In GC-MS (Gas Chromatography -Mass Spectroscopy ) analysis, the plant extract will first be…

Q: Explain the underlying physical principles in the creation of image contrasts for the following two…

A: Biological techniques are strategies for studying biological things. For biological research, they…

Q: We want to test different concentrations of an antibiotic to see how well it can kill bacteria on a…

A: Introduction:- The Agar dilution of the method is the currently the reference that standard the…

Q: Calculate the final dilution in the following dilution series:  a. 1 mL in  9 mL dilution & 100uL…

A: Dilution is calculated as amount of sample transfered into the diluent divided by the total amount…

Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…

A: This is the structure of Sulfamethoxazole.

Q: 2) Laura felt sick at a party last night after taking 3,4 methylenedioxymethamphetamine (MDMA),…

A: A drug is a chemical that, when it interacts with the body, has a physiological impact on it. They…

Q: You have a mixture of the three molecules shown below that you are using Gas Chromatography to…

A: Gas chromatography-mass spectroscopy is actually two techniques that are combined to form a single…

Q: A particular drug has first-order elimination rate kinetics and follows a one-compartment model.…

A: Animals and Humans ingest substances, drugs, or medicine, the body of the animal utilizes a variety…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: (8) Erythritol has been used for years as a reduced calorie sweetener (non-nutritive sweetener).…

A: Question 8: Erythritol Erythritol is a sugar alcohol (polyol) used as a reduced-calorie sweetener.…

Q: Calculate the dimensionless spray flux for the normal case and the atypical batch, and explain why…

A: As given in the question, the value of spray flux for: Normal value - 0.52 Atypical value - 1.46…

Q: A 56-year-old man comes to the clinic complaining of persistent fatigue for the past three months.…

A: Giveb data: A56-year-old man comes to the clinic with  complains of persistent fatigue for the…

Q: The Leica microscope that we will be using for histology lab has four different objective lenses and…

A: Microscopes are used to magnify an object. In biology laboratories compound light microscopes are…

Q: An iodine solution was sealed inside dialysis tubing, then immersed in a cup of water containing…

A: Dialysis membrane is a semipermeable membrane and allows only a few substances to pass on as…

Q: The basic components of a fluoroscopic x-ray system suitable for interventional procedures consists…

A: Fluoroscopy technique is an imaging technique that uses X-rays to obtain real-time moving images of…

Q: At what objective magnifications is it not OK to use the course focus adjustment? O A) 4X and 10X B)…

A: Microscope is an optical instrument which is used to enlarge and view microscopic objects.…

Q: Question 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution…

A: Given values: Initial culture cell concentration = 5×107 CFU/ml Final colonies on the plate = 50

Q: Why are the tubes sealed with a rubber stopper rather than left open to the atmosphere?

A: A rubber stopper is used to seal the tube opening as well as other laboratory glassware such as…

Q: When are the Korotkoff sounds loudest?

A: When a nurse or a health practitioner takes blood pressure through non invasive ways, they will be…

Q: Can you Identify your independent, dependent and controlled variables?

A: MALARIA- malaria is define as a disease which is caused by parasite known plasmodium. malaria is a…

Q: Lab students placed ten spinach leaf disks in each of three test tubes containing a baking soda…

A: Photosynthesis is the reaction by which the plant produces its food by utilizing carbon dioxide,…

Q: A sample was placed on a chromatography column. Dichloromethane was used as the eluting solvent. All…

A: Column chromatography is a technique that allows chemists to separate substances based on their…

Q: In hematology laboratory, automation has been the ongoing trend in terms of cell sorting and…

A: In hematology laboratory, automation performed as an improved accuracy of results and it has the…

Q: An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions.…

A: Number of colonies in the stock suspension = number of colonies plated * 1/ml* Dilution factor  here…

Q: Can all abnormal hemoglobins be diagnosed by electrophoresis? Explain your answer in detail.

A: The electrophoresis is a laboratory technique that uses the electro-kinetic process to separate the…

Q: Why is control of column and detector temperature more important for non- suppressed IC (Ion…

A: The control of column and detector temperature is a fundamental aspect of ion chromatography (IC), a…

Q: sample for WBC count was diluted by adding 20 µL of blood to 0.38 mL of diluting solution and then…

A: The white blood cell (WBC) count is an important diagnostic tool in healthcare, offering essential…

Q: You got an opportunity to join a professor lab who is working in-vivo model and specifically looking…

A: Mitochondria are called the power house of the cells as it is the site where the maximum amount of…

Q: Select the correct statement from the following option: O a. Spectroscopic methods require less time…

A: The branch of science that deals with the study of the interaction that can be described between the…

Q: The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions:…

A: UV-vis spectrophotometer A spectrophotometer is an instrument or apparatus that is used in the…

Q: lzz answer

A: Introduction: A living thing can be classified into many sorts based on its cells, which are life's…

Q: thanol has been carried over into your DNA sample (two answers possible here, select both of them)…

A: DNA is not soluble in ethanol . But when ethanol is there DNA gets separated by forming white…

Q: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test…

A: In order for the microorganisms to survive and grow under laboratory conditions we need to provide…

Q: 7) One of the following antibiotics doesn’t work through the same class of molecules as the rest: a)…

A: Daptomycin works through a different class of molecules known as lipopeptides, whereas the rest of…

Q: Can all abnormal hemoglobin be diagnosed by electrophoresis? Explain your answer in detail.

A: Electrophoresis means the migration of charged particles under the influence of an electric…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.