5. Why do we have to keep Fehling's A separate with Fehling's B?
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A:
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![5. Why do we have to keep Fehling's A separate with Fehling's B?
6. What is the chemical basis of Molisch test?
7. Explain for the disappearance of the color of iodine upon heating and its reappearance
upon cooling?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff9855b0c-bdd0-4bb7-abff-f6f58e761998%2Fdd499eef-83dc-48ab-872a-d1c9a4af3f86%2F3xzq44u_processed.jpeg&w=3840&q=75)
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- A certain amount of white solid was dissolved in enough water and gives a colorless solution. A portion of the solution was added with 15% solution of alpha-naphthol solution and a few drops of conc. H2SO4 forming a violet-colored ring. Another portion was added with drops of Seliwanoff’s reagent and showed no change after being warmed in a bath. Another portion was added with equal amounts of Fehling’s A and B reagent giving a red precipitate in a blue solution after warming in a bath. a) Cite an experimental result confirming that the solid is a carbohydrate b) Is the solid starch, fructose or glucose? Support your answer.3. The following absorbance spectrum was collected. What color is the sample solution? Explain using the color wheel. 1.4- 1.2 0.8 0.6+ 0.4 0.2 300 400 500 600 700 800 wavelength (nm) 4. Given the Beer's Law Plot for permanganate in the Introduction of the lab handout, what would be the concentration of an unknown permanganate sample if the absorbance was 0.308. Absorbance1. Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". A. Biuret Test B. Xanthoproteic Test C. Millon's Test D. Ninhydrin Test E. Hopkins-Cole Test F. Sulfur Test G. Heller's Test
- Provide a chemical test/reagent that would differentiate each of the following pairs of compounds. Also, for each test, indicate the compound (write only the corresponding CAPITAL LETTER) that would give the positive result, and the indication(s) for a positive result. ОН ОН 1. and A -CEC-H and 2. B HO CH3 OH 3. and B AExplain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)4. Complete the table for Watson-Schwartz Test Results for porphobilinogen and urobilinogen determination. Chloroform Layer (bottom) Modified Ehrlich Result Butanol Layer (top) Reaction Negative Urobilinogen positive Porphobilinogen positive Positive for other Ehrlich reactive substances
- A student carries out the following experiments and records the following observations. 1. A few drops of a solution of KOH (aq) is added to a solution of Fe(NO3)3 (aq). A red gelatinous solid forms immediately upon addition. 2. A few drops of a solution of KOH (aq) is added to a solution of NaNO3 (aq). No changes are observed. 3. A few drops of a solution of KOH (aq) is added to a solution that could contain Fe3+(aq) and/or Na+(aq) ions (the solution could contain both ions, just one ion, or neither ions). A red gelatinous solid forms immediately upon addition. Select all of the answers that are consistent with the data from those 3 experiments. Group of answer choices The unknown contains Fe3+(aq) and Na+(aq) The unknown contains Fe3+(aq) It is not possible to determine the presence of Na+(aq) from this experiment The unknown contains neither Fe3+(aq) or Na+(aq)1. Draw/illustrate the chemical reaction involved in Barfoed's test and explain the purpose of this qualitative test? 2. Draw/illustrate the chemical reaction involved in Seliwanoff's test and explain the purpose of this qualitative test? 3. Draw/illustrate the chemical reaction involved in Benedict's test and explain the purpose of this qualitative test? 4. Draw/illustrate the chemical reaction involved in Lugol's test and explain the purpose of this qualitative test?1. Acids and Bases a. How reliable are the indicators (red cabbage and phenolphthalein) in the testing of these household substances? b. Suggest how these tests be converted to specific (numeric) pH values.
- A) Refer to the image below to answer this question. You have a bacterial culture with a concentration of 4x10° bacterial cells per mL of broth. You borrow your initial culture tube to a fellow scientist and she performs the following dilutions: From the original culture 1 mL of broth was transferred to Flask A which contains 99 mL of sterile water. Then 1 mL from Flask A is transferred to Flask B and then 1 mL from Flask B is transferred to Flask C. buffer 1 ml 1 ml 1 ml A C 99 ml 99 ml 99 ml CULTURE Give the dilution of: Flask A Flask B Flask C 0.1 ml 0.1 ml Plate A Plate B Plate C 1.0 ml 1.0 ml Determine the dilution factor for the following questions: 1. What is the dilution from the original to Flask A? 0.1 ml 1 ml 0.1 mlA patient received a single 245.97 mg IV bolus dose of an antibiotic and the plasma drug concentration was determined as 13.62 mg/L and 5.94 mg/L at 2.04 hours and 11.52 hours following the drug administration, respectively. On a separate occasion, a single 427.73 mg oral dose of the same antibiotic was administered to the same patient and the plasma drug concentration was determined as 0, 5.01, 5.77, 3.43, 1.1 mg/L at 0, 3.15, 7.69, 14.39, and 27.5 hours following the drug administration. Please calculate the bioavailability of this drug without utilizing any data fitting program.1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.