Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Transcribed Image Text:5. In your own
words, explain how a standard curve is created and what purpose it serves.
Expert Solution
Step 1
A type of graph that is used for quantitative research for an assay is known as the Standard curve which is also known as a calibration curve. It is quantified as based on the measurements of multiple samples for that are known concentrations of the same properties to determine the unknown samples by interpolation on the graph. Thus the samples that are known concentrations for the same properties are known as standards and the graph is known as the standard curve.
E.g. Bradford assay.
|
Protein Concentration (µg/mL) |
Absorbance at 562 nm (%) |
|
0 |
0.001 |
|
125 |
0.011 |
|
250 |
0.03 |
|
375 |
0.062 |
|
500 |
0.092 |
|
625 |
0.119 |
|
750 |
0.169 |
|
875 |
0.218 |
|
1000 |
0.31 |
|
Unknown Sample |
Absorbance 562nm |
|
1 |
0.71 |
|
2 |
0.83 |
|
3 |
0.58 |
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