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a) A student performed a thin layer chromatographic separation experiment. In he first run, four standard substances A – azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In he second TLC run a mixture (X) of two of hese same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? b) A second mixture, Y, was run at the same АвсD ime. What is wrong with the solvent system

a) A student performed a thin layer chromatographic separation experiment. In he first run, four standard substances A – azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In he second TLC run a mixture (X) of two of hese same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? b) A second mixture, Y, was run at the same АвсD ime. What is wrong with the solvent system

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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10. (a) A student performed a thin layer chromatographic separation experiment. In the first run, four standard substances A - azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In the second TLC run a mixture (X) of two of these same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? (b) A second mixture, Y, was run at the same time. What is wrong with the solvent system used to try to separate Y? АвсD
Transcribed Image Text:10. (a) A student performed a thin layer chromatographic separation experiment. In the first run, four standard substances A - azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In the second TLC run a mixture (X) of two of these same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? (b) A second mixture, Y, was run at the same time. What is wrong with the solvent system used to try to separate Y? АвсD
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