Three sugars (Sugar A, Band C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. Aher running the chromatography and staining the following Retention factor () values were determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper. Sugar A, Rf= 0.23 Sugar B, Rf 0.65 Sugar C, Rf 0.27 What can be drawn from the results of this Paper Chromatography experiment? O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B. O Sugar B had the strongest interaction with the stationary phase among Sugars A, , and C. O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C. O Distance travelled by Sugar B must have been shorter than that of Sugars A and C. O Sugar B must be a non-reducing sugar given its high Rf value.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Three sugars (Sugar A, B and C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. After running the chromatography and staining, the following Retention factor (Rf) values were
determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper.
Sugar A, Rf = 0.23
Sugar B, Rf = 0.65
Sugar C, Rf = 0.27
What can be drawn from the results of this Paper Chromatography experiment?
O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B.
O Sugar B had the strongest interaction with the stationary phase among Sugars A, B, and C.
O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C.
O Distance travelled by Sugar B must have been shorter than that of Sugars A and C.
O Sugar B must be a non-reducing sugar given its high Rf value.
Transcribed Image Text:Three sugars (Sugar A, B and C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. After running the chromatography and staining, the following Retention factor (Rf) values were determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper. Sugar A, Rf = 0.23 Sugar B, Rf = 0.65 Sugar C, Rf = 0.27 What can be drawn from the results of this Paper Chromatography experiment? O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B. O Sugar B had the strongest interaction with the stationary phase among Sugars A, B, and C. O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C. O Distance travelled by Sugar B must have been shorter than that of Sugars A and C. O Sugar B must be a non-reducing sugar given its high Rf value.
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