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You will be making LB media in today's lab. Given the recipe for 1L of LB media on pg. 63 of this manual, determine how much of each ingredient you would need to make 150 mL of LB media. Show your calculations.

You will be making LB media in today's lab. Given the recipe for 1L of LB media on pg. 63 of this manual, determine how much of each ingredient you would need to make 150 mL of LB media. Show your calculations.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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• Sterile water OR sterile nutrient broth 8-1.5 mL sterile microcentrifuge tubes • P100 and P1000 micropipettes Hydrolytic Reactions Nutrient broth cultures of Bacillus subtilis, Escherichia coli and K. rhizorkila • Starch agar culture plate Casein agar culture plate Gram's iodine Endospore Staining Malachite green • Safranin • Steaming de-ionized water in 100ml beaker with boiling chips or beads • Piece of filter paper, 1x2 in • Bibulous paper Forceps / tweezers • Inoculation loop • Slide heater • Slide holder • Staining tray • Hotplate PROCEDURE Procedure A: Culture Medium Preparation You will prepare LB (Luria Broth) media, which is a standard complex media that is used to grow bacteria. 1. Determine mass of powder(s) to be used. Every litre (IL) of LB media contains the following: 10 g Bacto-tryptone • 5 g yeast extract 10 g NaCl **Each group of two students should make 150 mL of this media. Calculate the masses of reagents you will need. 2. Weigh out the reagent(s). Weigh the required amount of powder on a balance and add it to an empty beaker. 3. Add water. Add water to the beaker to a level just BELOW the final volume of your solution. (For example, if you want to make 150 mL of solution, you should add water to just BELOW the 150 mL mark on the beaker.) 4. Stir. Add a stir bar and stir until the powder complete dissolves in the water. 63
Transcribed Image Text:• Sterile water OR sterile nutrient broth 8-1.5 mL sterile microcentrifuge tubes • P100 and P1000 micropipettes Hydrolytic Reactions Nutrient broth cultures of Bacillus subtilis, Escherichia coli and K. rhizorkila • Starch agar culture plate Casein agar culture plate Gram's iodine Endospore Staining Malachite green • Safranin • Steaming de-ionized water in 100ml beaker with boiling chips or beads • Piece of filter paper, 1x2 in • Bibulous paper Forceps / tweezers • Inoculation loop • Slide heater • Slide holder • Staining tray • Hotplate PROCEDURE Procedure A: Culture Medium Preparation You will prepare LB (Luria Broth) media, which is a standard complex media that is used to grow bacteria. 1. Determine mass of powder(s) to be used. Every litre (IL) of LB media contains the following: 10 g Bacto-tryptone • 5 g yeast extract 10 g NaCl **Each group of two students should make 150 mL of this media. Calculate the masses of reagents you will need. 2. Weigh out the reagent(s). Weigh the required amount of powder on a balance and add it to an empty beaker. 3. Add water. Add water to the beaker to a level just BELOW the final volume of your solution. (For example, if you want to make 150 mL of solution, you should add water to just BELOW the 150 mL mark on the beaker.) 4. Stir. Add a stir bar and stir until the powder complete dissolves in the water. 63
You will be making LB media in today's lab. Given the recipe for 1 L of LB media on pg. 63 of this manual, determine how much of each ingredient you would need to make 150 mL of LB media. Show your calculations.
Transcribed Image Text:You will be making LB media in today's lab. Given the recipe for 1 L of LB media on pg. 63 of this manual, determine how much of each ingredient you would need to make 150 mL of LB media. Show your calculations.
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