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1. (a) An E. cofi DNA plasmid has 5.64 x 10 base pairs. The plasmid contains a single origin and a terminus, and replication rate is 1,000 base pairs per second. If the replication proceeds bidirectionally, how long would it take to replicate the whole plasmid? Show the steps clearly and give the answer in minute. (b) Lagging strand is formed from numerous broken fragments called Okazaki fragments. What factors guarantee that the numerous Okazaki fragments are assembled in a correct order in the lagging strand?

1. (a) An E. cofi DNA plasmid has 5.64 x 10 base pairs. The plasmid contains a single origin and a terminus, and replication rate is 1,000 base pairs per second. If the replication proceeds bidirectionally, how long would it take to replicate the whole plasmid? Show the steps clearly and give the answer in minute. (b) Lagging strand is formed from numerous broken fragments called Okazaki fragments. What factors guarantee that the numerous Okazaki fragments are assembled in a correct order in the lagging strand?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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5.64 x 10^6 base pairs Short questions
(a) An E. cofi DNA plasmid has 5.64 x 10 base pairs. The plasmid contains a single origin and a terminus, and replication rate is 1,000 base pairs per second. If the replication proceeds bidirectionally, how long would it take to replicate the whole plasmid? Show the steps clearly and give the answer in minute. 1. (b) Lagging strand is formed from numerous broken fragments called Okazaki fragments. What factors guarantee that the numerous Okazaki fragments are assembled in a correct order in the lagging strand?
Transcribed Image Text:(a) An E. cofi DNA plasmid has 5.64 x 10 base pairs. The plasmid contains a single origin and a terminus, and replication rate is 1,000 base pairs per second. If the replication proceeds bidirectionally, how long would it take to replicate the whole plasmid? Show the steps clearly and give the answer in minute. 1. (b) Lagging strand is formed from numerous broken fragments called Okazaki fragments. What factors guarantee that the numerous Okazaki fragments are assembled in a correct order in the lagging strand?
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