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. Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon at its normal chromosomal site and the regulatory se- quences only (laci, P laco) on a plasmid. Predict the effect of each mutation on the activity of B-galactosidase before and after the addition of inducer. Use -, +, or ++ to indicate approximate activity levels. Briefly explain the basis for each of your predictions. Mutation Before After (a) No mutations in either chromosomal or plasmid genes (b) A mutation in the plasmid operator, which abolishes its binding to repressor (c) A mutation in the chromosomal promoter, which reduces affinity of promoter for RNA polymerase by 10-fold (d) A lacI gene mutation in the chromosome, which abolishes binding of the lacl gene product to inducer (e) A lacī gene mutation in the plasmid, which abolishes binding of the repressor to the inducer (f) A chromosomal laco mutation, which abolishes its binding to repressor (g) A mutation in the gene for CRP, which abolishes its binding to cydlic AMP | | | |

. Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon at its normal chromosomal site and the regulatory se- quences only (laci, P laco) on a plasmid. Predict the effect of each mutation on the activity of B-galactosidase before and after the addition of inducer. Use -, +, or ++ to indicate approximate activity levels. Briefly explain the basis for each of your predictions. Mutation Before After (a) No mutations in either chromosomal or plasmid genes (b) A mutation in the plasmid operator, which abolishes its binding to repressor (c) A mutation in the chromosomal promoter, which reduces affinity of promoter for RNA polymerase by 10-fold (d) A lacI gene mutation in the chromosome, which abolishes binding of the lacl gene product to inducer (e) A lacī gene mutation in the plasmid, which abolishes binding of the repressor to the inducer (f) A chromosomal laco mutation, which abolishes its binding to repressor (g) A mutation in the gene for CRP, which abolishes its binding to cydlic AMP | | | |

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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. Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon at its normal chromosomal site and the regulatory se- quences only (laci, P laco) on a plasmid. Predict the effect of each mutation on the activity of B-galactosidase before and after the addition of inducer. Use -, +, or ++ to indicate approximate activity levels. Briefly explain the basis for each of your predictions.
Transcribed Image Text:. Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon at its normal chromosomal site and the regulatory se- quences only (laci, P laco) on a plasmid. Predict the effect of each mutation on the activity of B-galactosidase before and after the addition of inducer. Use -, +, or ++ to indicate approximate activity levels. Briefly explain the basis for each of your predictions.
Mutation Before After (a) No mutations in either chromosomal or plasmid genes (b) A mutation in the plasmid operator, which abolishes its binding to repressor (c) A mutation in the chromosomal promoter, which reduces affinity of promoter for RNA polymerase by 10-fold (d) A lacI gene mutation in the chromosome, which abolishes binding of the lacl gene product to inducer (e) A lacī gene mutation in the plasmid, which abolishes binding of the repressor to the inducer (f) A chromosomal laco mutation, which abolishes its binding to repressor (g) A mutation in the gene for CRP, which abolishes its binding to cydlic AMP | | | |
Transcribed Image Text:Mutation Before After (a) No mutations in either chromosomal or plasmid genes (b) A mutation in the plasmid operator, which abolishes its binding to repressor (c) A mutation in the chromosomal promoter, which reduces affinity of promoter for RNA polymerase by 10-fold (d) A lacI gene mutation in the chromosome, which abolishes binding of the lacl gene product to inducer (e) A lacī gene mutation in the plasmid, which abolishes binding of the repressor to the inducer (f) A chromosomal laco mutation, which abolishes its binding to repressor (g) A mutation in the gene for CRP, which abolishes its binding to cydlic AMP | | | |
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