. You have identified a mutation in yeast, a unicellular eukaryote, that prevents the capping of the 5′ end of theRNA transcript. However, much to your surprise, all theenzymes required for capping are normal. You determine that the mutation is, instead, in one of the subunitsof RNA polymerase II. Which subunit is mutant, andhow does this mutation result in failure to add a cap toyeast RNA?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
. You have identified a mutation in yeast, a unicellular eukaryote, that prevents the capping of the 5′ end of the
RNA transcript. However, much to your surprise, all the
enzymes required for capping are normal. You determine that the mutation is, instead, in one of the subunits
of RNA polymerase II. Which subunit is mutant, and
how does this mutation result in failure to add a cap to
yeast RNA?
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