How would transcription of the E. coli trp operon be affected by the following manip ulations of the leader region of the trp mRNA?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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 Transcription Attenuation
How would transcription
of the E. coli trp operon be affected by the following manip
ulations of the leader region of the trp mRNA?
(a) Increasing the distance (number of bases) between
the leader peptide gene and sequence 2
(b) Increasing the distance between sequences 2 and 3
(c) Removing sequence 4
(d) Changing the two Trp codons in the leader peptide
gene to His codons
(e) Eliminating the ribosome-binding site for the gene
that encodes the leader peptide
(f) Changing several nucleotides in sequence 3 so that
it can base-pair with sequence 4 but not with sequence 2

 

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