Reverse TranscriptionBlack-viral genomeIRNA(A)Green-tRNA from hostR US PBSPPTRed-minus strand DNA(-)ssDNA(-) Strand Synthesis(B)Blue-plus strand DNAPPTU3First Strand Transfer(C)PUGPPTU3 R(-) Strand SynthesisPPTUS R US(D)HIV ppt:PBSPPT5'-AAAAGAAAAGGGGGG-34(-) & (+) Strand Synthesis(E)PPT(+) Strand SynthesisPPTUS R US(F)Second Strand Transfer(+)ssDNAPBS(G)U3 R USPBS(H)Strand displacement synthesisUS R US PBSPPTU3 R USUS R US PBSLTRPPTLTRBasu et al., Virus Res. 2008, 134:19-38.Use the image above to answer the multiple choicequestion: If an infection with HIV is carried out with avirus that has a mutations that eliminates the RNase Hactivity of reverse transcriptase, which of the followingwould be observed?No viral DNA would be observedComplete viral double stranded DNA would be observed.Only a small piece of minus sense DNA would be observedNearly completed minus sense but no plus sense DNA wouldbe observed.Nearly full length minus sense DNA but just a small piece ofplus sense DNA would be observed.

In an HIV infection caused by a virus with mutations that eliminate reverse transcriptase's RNase H…

Answered: Reverse Transcription Black-viral…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 17P

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5’-GGC TAC GTA ACT TGA TAA-3’ (a) mRNA codons that are transcribed from the DNA (b) tRNA anticodons for each of the mRNA codons (c) The sequence of amino acids in the resulting polypeptide. (d) Provide the sequence of another possible DNA strand that will lead to synthesis ofthe same polypeptide.
nd 2 minutes): Any RNA polymerase in any organism: O A Synthesizes RNA chains in the 3 to-5" direction O B. Binds tightly to a reqion of DNA located thousands of base pairs away from the transcobed rogion of the DNA OC Has proofreading activity O D. Separates DNA strands throughout a long region of DNA (up to tinousands of base pairs) and then copies one of them. OE Has a subunit called A (lambda), which acts as a proofreading ribonuclease OF. Can initiate synthesis of a new RNA chain without a primer
of estion 9 t of uestion THCA ▶ Sou 100- HC This reaction is Entropy is THC San Leafly ATA RNA polymerase SSSSSSSSSS ATTOGOGACATAA ATGACGGATCAGCCOCAAG UACUOCCUAGUC RNA Transcript TACTOCCTAGTCGGCOTTCOOCTTAACCOCTOTATIT (In this picture, RNA is being made by complementary base pairing with DNA.) This reaction is → Entropy is ◆
ANALYSIS 1: Given the following sequence of bases present in the DNA coding strand, what will be the sequence on the template strand, on the MRNA strand and on the peptide? Show the sequence of amino acids if A is inserted between C-6 and T-7. (5') CTT-AGC-TGG-CCC... (3') Key-in your answers in this manner: template strand (3') XXX-XXX-XXX-XXX (3') MRNA strand (5') XXX-XXX-XXX-XXX (3) Wild-type peptide AA1-AA2-AA3-AA4 Mutant peptide AA1-AA2-AA3-AA4 2 buse n codon A. Phe Phe Sir Lni tase ne uopes n s
Please label the following: (A) INITIATION *Complementary strand *Template Strand *RNA polymerase *Initiation Site *Termination Site *Promoter *5' and 3' end on each DNA strand Initiation (A) FRA FORÆTSIST Elongation (B) (C) restorag Termination IND PLATIN RNA (B) ELONGATION *Exiting DNA *Exiting RNA Transcript *Template Strand *Nucleotides (ATP, UTP, CTP, GTP) *Direction of transcription PHILLI 13' DIDI
Pues (two-ringed) 9. (a) Label each nitrogenous base in the double strand of DNA in Figure 4. NEL P-C.₂. H- (a) -CH₂-P-C₂ 5' 0 PCH₂ (b) -Н -CH₂-P-C₂ 5' 0 PCH₂ CH₂-P-C₂ H 0 I' P 5' 0 (d) 3' CH₂ H PCH₂ Figure 4 (b) Figure 4 above shows a phosphodiester bond. Explain what this is. 1.5 Pr
5'– ATGGCGAGGCGGCAGCTGTTATGGTGA – 3' In the sequence above, suppose that the 20th nucleotide of the template (an T) was mutated to a A. (A) Now, what is the mRNA sequence? (B) What is the amino acid sequence of the translated protein? (C) Would this protein be able to carry out its function?
5' UGG CAA UCC UAC GAU 3' - 1. Here is the MRNA sequence from a section of a gene (it is the middle of the sequence, so it has no AUG). What is the template sequence of this gene? - 2. Are any of these codons in the MRNA non-degenerate? If so, indicate which one. e 3. 4 a) Translate this mRNA section. Give the 3 letter codes for the amino acids. b) Indicate on the peptide which is the C terminus and which is the N terminus. e 4. Is it possible for a single base pair substitution to cause a truncation in this peptide? If so, e explain how. e 5. Write out the sequence of the anticodon in the tRNA that would bind to the fourth codon in the e MRNA. e 6. Write out a possible miRNA that could regulate the expression of this gene
Provide a brief description behind your choice? Virus-mediated transfer of cellular genetic material from one bacterial cell to another by means of virus particles is called: (A) transduction (B) transposition (C) transformation (D) transfection One strand of double-stranded DNA is mutated, changing all cytosines to uracils. After one round of replication of the mutated DNA strand, the melting temperature of the resulting DNA will: (A) be higher (B) be lower (C) remain the same (D) be double The Southern blotting technique is used for: (A) the detection of RNA fragments onmembranes by specific radioactiveantibodies (B) the detection of DNA fragments onmembranes by a radioactive DNAprobe (C) the detection of proteins on membranesusing a radioactive DNA probe (D) the detection of DNA fragments onmembranes by specific radioactiveantibodies Superoxide dismutase is an important enzyme for maintenance of red blood cells and is defective insome neurodegenerative diseases. What…
Multiple Replication Forks in E. coli II On the basis of Figure 28.2, draw a simple diagram illustrating replication of the circular E. coli chromosome (a) at an early stage, (b) when one-third completed, (c) when two-thirds completed, and (d) when almost finished, assuming the initiation of replication at oriC has occurred only once. Then, draw a diagram showing the E. coli chromosome in problem 3 where the E. coli cell is dividing every 20 minutes.
5. Strand1 Direction of 3' unwinding Strand 2 b The diagram shows a replication fork. i. What is the polarity of a Which strand is the template for the leading strand? Which strand is the template for the lagging strand? On which strand are Okazaki fragments made? ii. i. iv. 6. Below represents a promoter in a transcription unit ----TATAAT-strand 1 ---ATATTA--strand 2 Fill in the polarity of the strands? Which strand is the coding strand? Which strand is the template strand? What is the direction of transcription? 7. Below represents a promoter in a transcription unit -----TAATAT - -----ATTATA--- Fill in the polarity of the strands? Which strand is the coding strand? Which strand is the template strand? What is the direction of transcription?
EcoRI --- 5' G - AATTC 3' 5' AGAATTCCGACGTATTAGAATTCTTAT CCGCCGCCGGAATTCT CATCA 3' 3' TCTTAAGGCTGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 5' Number of pieces of DNA , and type of fragment .

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