How did the following in-vitro transcription assay utilizing the rrnB promoter help elucidate the UP story? Explain the experiment, the controls [lacUV5, RNA-1], the various DNA promoters, and the data [including any artifact]. Gel A..... gel B RNAP (a) lacUV5- mm P1 RNA-1 Copyright The McGraw-H Companies, Inc. Permission required for reproduction or display (b) (c) WT R265C WT Wild-type lac -88 SUB-41 UVS vector a-235 lac -88 SUB-41 UVS vector lacUVS- 8P1- RNA-1- 8265C WTR265C -88 SUB-88 SUB lacuVS 18:11 12345678910 12345678910 1234567891011121314 O Ross et al, A hird recognition alement in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase Science 262 26 Nov 1993) 1. 2, p. 1408 0 AAAS
How did the following in-vitro transcription assay utilizing the rrnB promoter help elucidate the UP story? Explain the experiment, the controls [lacUV5, RNA-1], the various DNA promoters, and the data [including any artifact]. Gel A..... gel B RNAP (a) lacUV5- mm P1 RNA-1 Copyright The McGraw-H Companies, Inc. Permission required for reproduction or display (b) (c) WT R265C WT Wild-type lac -88 SUB-41 UVS vector a-235 lac -88 SUB-41 UVS vector lacUVS- 8P1- RNA-1- 8265C WTR265C -88 SUB-88 SUB lacuVS 18:11 12345678910 12345678910 1234567891011121314 O Ross et al, A hird recognition alement in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase Science 262 26 Nov 1993) 1. 2, p. 1408 0 AAAS
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Molecular biology, please explain in detail
![- How did the following in-vitro transcription assay utilizing the rrnB promoter help elucidate the UP story? Explain
the experiment, the controls [lacUV5, RNA-1], the various DNA promoters,
and the data [including any artifact].
(a)
Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
(b)
(C) WT
Wild-type
RNAP
a-235
R265C WT R265C WTR265C
lac
-88 SUB-411 UVS vector
-88 SUB-41 UV5 vector
-88 SUB-88 SUB lacUVS
lacUV5-
lacuV5-
rm8 P1-
RNA-1-
Gel A.....
1 2 3 4 5 6 7 8 9 10
1 2 3 4 5 6 7 8 9 10
123456 7 8 9 10 11121314
O Ross et al, A third recognition alement in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase
"Science" 262 (26 Nov 1993) 1. 2. p. 1408 O AAAS
gel B](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F803d528b-eb2c-48e5-96de-27076df17871%2F0ad8dbdb-060d-4c71-9bb2-077ee10ae35e%2Fri51gm_processed.jpeg&w=3840&q=75)
Transcribed Image Text:- How did the following in-vitro transcription assay utilizing the rrnB promoter help elucidate the UP story? Explain
the experiment, the controls [lacUV5, RNA-1], the various DNA promoters,
and the data [including any artifact].
(a)
Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
(b)
(C) WT
Wild-type
RNAP
a-235
R265C WT R265C WTR265C
lac
-88 SUB-411 UVS vector
-88 SUB-41 UV5 vector
-88 SUB-88 SUB lacUVS
lacUV5-
lacuV5-
rm8 P1-
RNA-1-
Gel A.....
1 2 3 4 5 6 7 8 9 10
1 2 3 4 5 6 7 8 9 10
123456 7 8 9 10 11121314
O Ross et al, A third recognition alement in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase
"Science" 262 (26 Nov 1993) 1. 2. p. 1408 O AAAS
gel B
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