EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 6MC
Summary Introduction
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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What is the enzymatic function of restriction enzymes?
Group of answer choices
a. to cut nucleic acids at specific sites
b. to join nucleotides during transcription
c. to add new nucleotides to the growing strand of DNA
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If you are a genetic engineer and you cloned your gene of interest in a plasmid and you
want to know if the protein encoded by the cloned gene is expressed or not, which of
the following methods is the right one to use?
Select one:
a. Northern blot
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c. Agarose gel with polyacrylamide
d. Western blot
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What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases?
a.
PCR product of a gene is different from the expected one
b.
The size of a recombinant DNA is different from the expected one
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Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
- Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forwardWhich of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translationarrow_forwardSome bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: Select one: O a. DNA polymerases O b. DNA ligases c. restriction endonucleases O d. restriction ligasesarrow_forward
- The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardIf a restriction endonuclease recognizes and cleaves a linear piece of DNA and Circular DNA at 8 distinet places, how many pieces will result? OA Nine and Eight fragments respectively B. Both gerierate nine fragments C. Ten and nine fragments respectively Both generate eight fragmentsarrow_forwardThe restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forward
- Why do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable bacteria to repair lesions in its DNA B. Restriction enzymes enable prokaryotes to assimilate genes or fragments of DNA from its environment C. Restriction enzymes excise phage DNA from prokaryotes thereby protecting them from lysis. D. Restriction enzymes help unwind DNA and facilitate DNA replication in prokaryotes E. Restriction enzymes are necessary for editing DNA during replication.arrow_forwardWhich of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA b. AAGCTTCGAA c. GAATTC d. ACCATTGGAarrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forward
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