EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 4MC
Summary Introduction
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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What is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3
Which of the following is a concern of in-vivo gene therapy?
A. Lytic vector expression
B. Integration into target region
C. Immune response to viral vector
D. Production of chimera
When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- A graduate student isolates two new retroviruses: Virus A and Virus B. To determine whether these viruses could cause cancer, the student infected human cells with each of the viruses and then transfered the infected cells to nude mice to see if the viruses would cause formation of tumors. The student was able to determine by PCR that both viruses are integrated into the genome of the host cells before transplanting cell in mice. The data for this assay are shown in the figure below. Explain how the mechanism of transduction may be different for these two viruses to yield the observed data (limit 4-5 sentences). Retrovirus Transformation Assay % Cells Cause Tumors 120 100 80 60 40 20 0 0 10 -Virus A -Virus B 30 20 Days Post Infection 40arrow_forwardWhat is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.arrow_forwardDiscuss the four important features of DNA vectors.arrow_forward
- CHOICES: a. Plasmid b. Sticky end c. DNA ligase d. Transformation e. Restriction enzyme f. Genetic marker g. Transduction QUESTIONS: Cuts the DNA into fragments Circular DNA molecule of bacteria Used to insert DNA of interest to vector Area of DNA where bases are ready to be paired Recombinant DNA technology with the help of a vector genearrow_forwardWhich one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0arrow_forwardAmong the following statements regarding the use of viral vectors, which is (are) true: (can be more then one answer) A.Viral vector transfection has the defect of rapidly killing cells, given the high toxic potential of viruses. B.Generally, the effectiveness of a viral infection transfection is much higher than an electroporation or cationic liposome transfection. C.Even before infecting a cell, a virus can begin to express the indicators, accelerating the process of expression of the indicator in the culture medium. D.Adeno-associated viruses are little used in biophotonics, as they require simultaneous adenovirus infection.arrow_forward
- If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know if the protein encoded by the cloned gene is expressed or not, which of the following methods is the right one to use? Select one: a. Northern blot b. Both Northern and Western blots c. Agarose gel with polyacrylamide d. Western blot e. Protein gel and northern blotarrow_forwardA has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forward4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for the enzyme glutathione-S-transferase (GST) is used in purifying your protein of interest. Briefly explain how this is done. 6) What enzyme is used to create CDNA from MRNA?arrow_forward
- The CRISPR-Cas9 system can be used to: a. sequence a genome in living cells b. edit a gene in living cells and organisms c. diagnose sickle-cell anemia d. identify a specific gene e. detect gene expression under different conditionsarrow_forwardThe other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardWhich of the following is NOT common to all repair mechanisms? A. Detection of the lesion B. Removal of the damaged DNA sequence/ nucleotide/ base C. Removal of free radicals at the site of injury D. Repair of the lesion E. Involvement of enzymes in removing the lesion and/or repair of the damaged sequence/nucleotide/base.arrow_forward
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