Introduction The gel electrophoresis method is used to separate the genetic material or PCR amplified products based on their molecular size, shape, and electrical charge. This technique helps to identify and isolate genes of interest or DNA fragments. Such DNA sequences are used to insert into donor organisms through vectors to create genetically modified organisms (GMOs) or serve as a template for PCR amplification or construction of the gene library. The electrophoresis technique is involved to evaluate PCR samples for clinical or genetic diagnosis. Also, it is used for the separation of restricted DNA or RNA samples before transfer in Southern blotting or Northern blotting, respectively.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 5MTF

Summary Introduction

Introduction

The gel electrophoresis method is used to separate the genetic material or PCR amplified products based on their molecular size, shape, and electrical charge. This technique helps to identify and isolate genes of interest or DNA fragments. Such DNA sequences are used to insert into donor organisms through vectors to create genetically modified organisms (GMOs) or serve as a template for PCR amplification or construction of the gene library. The electrophoresis technique is involved to evaluate PCR samples for clinical or genetic diagnosis. Also, it is used for the separation of restricted DNA or RNA samples before transfer in Southern blotting or Northern blotting, respectively.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Fill in the blank 1. A genetically engineered rodent in which a normal gene has been replaced by a a defective version of the same gene is called ______. 2. DNA fingerprints (ex: in forensics) can be generated by selectively cutting DNA with bacterially derived proteins called _______ and examining the resulting DNA fragment patterns on agarose gels.

Activity 2. You COMPLETE Me! Objective: Identify the methods used for inserting plasmids into the host cells. What you need: pen and paper What to do: On a separate sheet of paper, copy and complete the key points of the methods used to introduce plasmids into the host cells. Choose your answers from the box gene gun biolistics electric shock mammalian cells plasmid insertion by Heat Shock Treatment increase the pore sizes of their plasma membranes (2) competent plasmid DNA Heat Shock electroporation There are three methods on introducing plasmids into the host cells namely: (1)_ (3) Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues. Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a The pretreatment is said to make the cells (6). pretreatment procedure to (5) the introduction of the (7)_ plasmid at about 4°C for about 30 minutes and the plasmids concentrate near the cells during After the pretreatment, the cells are incubated with…

I need the answer as soon as possible