Concept explainers
To answer:
Recognition and restriction of DNA sequence by HhaI.
Introduction:
Restriction enzymes recognize and cut DNA
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EBK MICROBIOLOGY:W/DISEASES BY BODY...-
- Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?)arrow_forwardThis is part of the Escherichia coli DNA sequence that contains an inverted repeat. (Note: top strand is the coding strand). 5'-AACGCATGAGAAAGCCCCCCGGAAGATCACCTTCCGGGGGCTTTATATAATTAGC-3' 3'-TTGCGTACTCTTTCGGGGGGCCTTCTAGTGGAAGGCCCCCGAAATATATTAATCG-5' (i) Draw the structure of hairpin loop that will be formed during the end of transcription. (ii) Describe the function of the hairpin loop during transcription.arrow_forwardDNA is driven into the A-form when under dehydrating conditions. Describe the differences between A-form and B-form nucleic acid in the major groove and minor groove.arrow_forward
- A short protein segment was cleaved by two different enzymes resulting in two sets of degradation products with multiple fragments in each set. The amino acid sequence of each fragment was determined via Edman degradation. The fragments obtained from Enzyme 1 have the following sequences: LLIGVVQT RKNS SFWAA EED Meanwhile, the fragments obtained from Enzyme 2 were found to have the following sequences: QTEED SSFWAALL IGVV RKN What is the amino acid sequence of the short protein segment from which these fragments were derived? O RKNSSFWAALLIGVVQTEED O LLIGVVQTSFWAARKNSEED O SSFWAALLQTEEDIGVVRKN O IGVVRKNSSFWAALLQTEEDarrow_forwardList the sequences of RNA that would be transcribed from the following DNA template sequences. TTACACTTGCTTGAGAGTC ACTTGGGCTATGCTCATTA GGCTGCAATAGCCGTAGAT GGAATACGTCTAGCTAGCAarrow_forwardDirect repair of pyrimidine dimer formation in E. Coli can be accomplished by nucleotide excision. true or false?arrow_forward
- Identify the dinucleotide CA repeat region and the score in the following sequence:TGGCACACTCACACCACACAGACAGTTAarrow_forwardIs there any situation in which DNA is made based on a RNA template? If there is,explain with an example how it occurs and state the enzyme involved?arrow_forwardA DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Lengtharrow_forward
- The enzyme dihydrofolate oxidase has 3 adjacent asparagines residues (codon for asparagines is ACC): explain how site directed mutagenesis could be used to increase the thermal stability of the proteinarrow_forwardA DNA fragment with the following base sequence has some cytosine bases that are methylated (indicated by C*) and others that are unmethylated. To determine the locations of methylated and unmethylated cytosines, researchers sequenced this fragment both with and without treatment with sodium bisulfite. Give the sequence of bases that will be read with and without bisulfite treatment. —ATCGC*GTTAC*GTTGC*GTCA—arrow_forwardProvide the sequences of the template and coding strands of a DNA double helix that was used to produce this RNA: 5'-AUUACGGUCUAU. Be sure to label the 5' and 3' ends.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning