EBK MICROBIOLOGY:W/DISEASES BY BODY...-
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 6CT
Summary Introduction

To answer:

The number of DNA molecules synthesized in PCR reaction using 15 DNA helices in 15 cycles.

Introduction:

PCR technique is a rapid method used to amplify the huge amount of a specific segment of the DNA molecule. It is used to generate a billion copies of identical molecules of the DNA segment within few hours. It is used to analyze genomic variation and mainly involved in clinical studies. The important components used for PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. There are nearly 30 cycles are involved in PCR to produce numerous copies of exact replicas.

The main steps of PCR in each cycle:

Denaturation: Separation of double-stranded DNA into two single-strand DNA molecules by cleaving the hydrogen bonds at 94°C.

Annealing: Binding of primers (20 - 30 nucleotides long) to ends of ssDNA molecules at 65°C.

Extension: Addition of dNTPs at 3’ site of the primer by DNA polymerase to synthesize complementary stand of ssDNA at 72°C.

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Cellular DNA replication uses the enzymes for the process while PCR uses thermal denaturation that helps seperate DNA strands.  I need a picture that shows the difference between them two using the statement above.
The way PCR amplifies DNA is similar to the doubling in apopulation of growing bacteria—a single DNA strand is used tosynthesize 2 DNA strands, which become 4, then 8, then 16, etc. If acomplete cycle takes 3 minutes, how many strands of DNA wouldtheoretically be present after 10 minutes? After 1 hour?
Order the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of DNA to be sequenced add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size The DNA sequence is determined denature the double-stranded DNA Answer Bank
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