EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 10MC
Summary Introduction
Introduction:
DNA microarray is composed of single-stranded DNA molecules (ssDNA) as cDNA or genomic DNA, which are attached to the solid substrate, such as glass slide, a nylon membrane, or silicon chips. It is also called DNA chip as it contains a collection of thousands of various DNA molecules at a specific site on the array. The single array may contain DNA molecule from one or more species. The fluorescent ssDNA molecules are used as labels, which bind to their complementary sequence of DNA on the array. This technique is used to find DNA or RNA in a sample and to evaluate the expression of genes.
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DNA can be separated based on fragment size using ________.
a.
restriction enzymes
b.
gel electrophoresis
c.
PCR
_____ cut(s) DNA molecules at specific sites. a. DNA polymerase c. Restriction enzymes b. DNA probes d. Reverse transcriptase
____ cut(s) DNA molecules at specific sites.
a.
DNA probes
b.
Restriction enzymes
c.
DNA polymerase
d.
DNA ligase
Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- _____ can correct a genetic defect in an individual. a. Cloning vectors c. Sequencing b. Gene therapy d. Electrophoresisarrow_forwardPlease explain right answerarrow_forwardChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forward
- Enzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forwardRFLP analysis involves the following, except ________ . a. more than one restriction sites b.gene therapy c.intercalation of dye d.electrophoresis e.restrictionarrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forward
- Features of restriction enzymes include the following, except __________ . a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNAarrow_forwardWhich of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forward
- The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardWe want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNAarrow_forwardWhich of the following objects would you use microinjection in order to transfer DNA into? a. Egg cell visible to the human eye b. Animal virions c. Escherichia coli that has an F plasmid d. Bacteria that has been heat shockedarrow_forward
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