Concept explainers
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Why did the discovery and development of restriction enzymes speed up the study of recombinant DNA technology.
Introduction:
The recombinant DNA technology involves isolation and alteration of genetic materials to produce genetically modified organism (GMO). This technology isolates DNA from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The important tools used to create genetically changed DNA or organisms are
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EBK MICROBIOLOGY:W/DISEASES BY BODY...-
- Why is the use of a heat-stable DNA polymerase important to the success of PCR?arrow_forwardBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.arrow_forwardWhat are some safety and ethical concerns raised by the use of recombinant DNA technology? What are some dangers of introducing genetically engineered organisms into the environment?arrow_forward
- What occurs during the HIGH temperature step of PCR? A) DNA is cut by restriction endonucleases B) primers get degraded C) primers anneal to single-stranded DNA D) DNA is synthesized E) double stranded DNA separates into single stranded DNAarrow_forwardChoose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)arrow_forwardAssuming you started with only one copy of a DNA sequence, how many copies should be present after 15 cycles of PCR?arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning