EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 1SA
Describe three artificial methods of introducing DNA into cells.
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Describe the whole process and the principle behind DNA extraction.
b) Describe how DNA is digested by different restriction enzymes
c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
DEFINE THE FOLLOWING:
1) restriction enzyme
2) plasmid
3) recombinant DNA
Chapter 8 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Identify two problems associated with genetically modified organisms.arrow_forwardDiagram the steps for the production of recombinant DNA. Label and Identify the steps involved and the protein/enzyme requirement for each.arrow_forwardBriefly explain about computer-automated high-throughput DNA sequencing ?arrow_forward
- Describe the processes involved in denaturing and renaturing of DNA, and explain what is useful about this procedure.arrow_forwardDescribe various ways in which the sequence of DNA is verified or corrected. Why are there so many pathways?arrow_forwardDescribe the key steps in the semiconservativereplication of DNA.arrow_forward
- Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the number of nucleotides incorporated per chain per polymerase binding event).arrow_forwardHow is DNA amplified in a polymerase chain reaction(PCR) procedure?arrow_forwardDescribe some of the more commonly used methods to introduce mutations into DNAarrow_forward
- Outline four key steps involved in producing a recombinant DNAarrow_forwardWhich of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve DNA strand cleavage by an endonuclease. Both NER and BER can be activated by exposure to visible light. Only BER requires DNA polymerase. Both NER and BER involve the creation of an apyrimidinic (AP) site.arrow_forwardWhich of the following statements about DNA polymerase I are true? 1) It requires both template DNA and a primer. 2) It can copy and join the ends of single-stranded circular DNA. 3) It uses only DNA as a template primer. 4) With respect to linear DNA, it copies only the 5′ end and then dissociates. a) Only statements 1 and 3 are true. b) Only statements 1 and 4 are true. c) Only statements 1, 2, and 4 are true. d) All of the listed statements are true.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license