Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 20P
The inhibitory effect of an uncompetitive inhibitor is greater at high [S] than at low [S]. Explain this observation.
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The inhibitory effect of an uncompetitive inhibitor is greater at high [S] thanat low [S]. Explain this observation.
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why is non-competitive inhibiton a special type of mixed inhibiton?
Chapter 8 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 8 - Prob. 1PCh. 8 - The enzyme urease catalyzes the hydrolysis of urea...Ch. 8 - An enzyme contains an active site aspartic acid...Ch. 8 - The folding and unfolding rate constants for a...Ch. 8 - In some reactions, in which a protein molecule is...Ch. 8 - Would you expect an “enzyme” designed to bind to...Ch. 8 - The initial rate for an enzyme-catalyzed reaction...Ch. 8 - a. If the total enzyme concentration in Problem 7...Ch. 8 - Prob. 9PCh. 8 - Prob. 10P
Ch. 8 - The following data describe the catalysis of...Ch. 8 - At 37 oC, the serine protease subtilisin has kcat...Ch. 8 - The accompanying figure shows three...Ch. 8 - The steady-state kinetics of an enzyme are studied...Ch. 8 - The same enzyme as in Problem 14 is studied in the...Ch. 8 - Enalapril is an anti-hypertension “pro-drug"...Ch. 8 - Initial rate data for an enzyme that obeys...Ch. 8 - Prob. 18PCh. 8 - Suggest the effects of each of the following...Ch. 8 - The inhibitory effect of an uncompetitive...Ch. 8 - Prob. 21PCh. 8 - Prob. 22PCh. 8 - Prob. 23PCh. 8 - In kinetics experiments, the hydrolysis of the...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- The nerve gas sarin acts as a poison by covalently bonding to a hydroxyl group in the active site of the enzyme acetylcholinesterase. This binding results in a higher-than-normal amount of acetylcholine at a nerve synapse, resulting in muscle spasms. From this description, would you expect sarin to be a competitive, noncompetitive, or irreversible inhibitor? noncompetitive inhibitor irreversible inhibitor competitive inhibitorarrow_forwardAn Eadie-Hofstee plot is shown below for the different types of enzyme inhibition. Match the line withthe type of inhibition.(a) Line A Inhibition Mechanism. Ans: ________(b) Line B Inhibition Mechanism. Ans: ________(c) Line C Inhibition Mechanism. Ans: ________arrow_forwardWhat type of inhibition is being shown in the following graphs?arrow_forward
- When we compare the binding of I and of S to the enzyme in a mixed noncompetitive inhibitor, we assumed that the binding of I decreased the affinity of the enzyme for S. What would happen if the opposite were true?arrow_forwarddescribe the mechanism of each class of inhibitor, including how they impact the effective concentrations [E] and/or [ES] and where they bind on the enzyme:arrow_forwardIn pure noncompetitive inhibition: a. Where on the enzyme does the inhibitor bind? b. Does the inhibitor bind to E, ES or both? c. What is the effect of I on Vmax? d. What is the effect of I on Km?arrow_forward
- Using equilibrium argument, why does Km apparently increase, decrease or stay the same in uncompetitive inhibition?arrow_forwardWrite a balanced equation for the hydrolysis of cyclic GMP, catalyzed by CGMP phosphodiesterase. Would you expect an inhibitor of this enzyme to potentiate or antagonize the action of Viagra? Explain.arrow_forwardInhibitors of acetylcholinesterase, such as edrophonium, are used to treat Alzheimer’s disease. The substrate for acetylcholinesterase is acetylcholine. Structures are attached. What kind of inhibitor is edrophonium? Explain. Can inhibition by edrophonium be overcome in vitro by increasing the substrate concentration? Explain. Does this inhibitor bind reversibly or irreversibly to the enzyme? Explain.arrow_forward
- Write a balanced equation for the hydrolysis of cGMP, catalyzed by cGMP phosphodiesterase. Would you expect an inhibitor of this enzyme to potentiate or antagonize the action of Viagra? Explain.arrow_forwardUDP-glucuronosyltransferase enzymes bind the organic compound UDP-glucuronic acid (UDP-GA) in order to catalyse the transfer of a glucuronic acid group from UDP-GA to a drug molecule, releasing UDP from the active site as a product. UDP is then regenerated by the activity of another enzyme. What terms could be used to describe UDP-GA?arrow_forwardWhat is the relative inhibition of an enzyme by a competitive inhibitor at [S] = KS and [I] = KI?arrow_forward
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