Concept explainers
(a) Interpretation:
Role of glu, tyr and asp should be identified.
Concept introduction:
Glutamine can also be denoted by E 117 and act as General acid catalyst in 1st step and helps in the catalysis of acid whereas it act as General base catalyst in another step.
Glutamine is consist of carboxyl group which attacks the hydroxyl of sialic acid and thus it act as a base catalyst and then it turns into carboxyl group whereas aspartic acid contains amino group which looses electrons to sialic acid and behaves as base.
Nuraminidase is an enzyme helps in hydrolysis of sialic acid which is present on cell surface as binded protein.
(b) Interpretation:
Effect of mutation of glu to ala on Km should be identified.
Concept introduction:
Glutamine can also be denoted by E 117 and act as General acid catalyst in 1st step and helps in the catalysis of acid whereas it act as General base catalyst in another step.
Glutamine is consist of carboxyl group which attacks the hydroxyl of sialic acid and thus it act as a base catalyst and then it turns into carboxyl group whereas aspartic acid contains amino group which looses electrons to sialic acid and behaves as base.
Nuraminidase is an enzyme helps in hydrolysis of sialic acid which is present on cell surface as binded protein.
(c) Interpretation:
How R374 stabilises the transition state should be identified.
Concept introduction:
Glutamine can also be denoted by E 117 and act as General acid catalyst in 1st step and helps in the catalysis of acid whereas it act as General base catalyst in another step.
Glutamine is consist of carboxyl group which attacks the hydroxyl of sialic acid and thus it act as a base catalyst and then it turns into carboxyl group whereas aspartic acid contains amino group which looses electrons to sialic acid and behaves as base.
Nuraminidase is an enzyme helps in hydrolysis of sialic acid which is present on cell surface as binded protein.
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Biochemistry: Concepts and Connections (2nd Edition)
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