Concept explainers
(a) Interpretation:
Role of Glu 270 in catalysis in the mechanism of carboxypeptidase A should be identified.
Concept introduction:
Glutamine can also be denoted as Glu 270.It is a general base catalyst in 1st step and helps in the catalysis of base whereas it act as general acid catalyst in another step.
Glutamine consists of carboxyl group which attacks the hydroxyl of Zn2+ and thus, it act as a base catalyst that turns into carboxyl group whereas arginine contains ammonium ion which helps in the ionic interaction with the carboxypeptidase.
Carboxypeptidase is an enzyme helps in hydrolysis of peptide of proteins and thus converting trypsinogen to trypsin and chymotrypsinogen to chymotrypsin.
(b) Interpretation:
Role of R 145 in catalysis in the mechanism of carboxypeptidase A should be identified.
Concept introduction:
Arginine having a molar mass of 145contains amino group and ammonium ion. With the help of ammonium ion, the arginine can ionize the substrate by forming a bond with carboxyl group.
Want to see the full answer?
Check out a sample textbook solutionChapter 8 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON