Chapter 8, Problem 17P

Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table. When the enzyme concentration is 3 nmol ml-1, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.00426 (µmol- ml s).

1. Calculate k_cat for the reaction.
2. Calculate K_M for the enzyme.
3. When the reactions in part (b) are repeated in the presence of 12 µM of an uncompetitive inhibitor, the y-intercept of the Lineweaver-Burk plot is 0.352 (µmol-1 ml s). Calculate K^’_I for the inhibitor.