Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 4, Problem 8TYU
Summary Introduction

Introduction:

Quantification of nucleic acids is generally used in molecular biology to determine the average concentrations of DNA (Deoxyribose nucleic acid) or RNA (Ribose nucleic acid) present in a sample, which further assists in determining the purity level of nucleic acids. In general, the ratio of the adenine is same as the thymine and the ratio of the guanine is same as the cytosine.

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The absorbance of a nucleic acid sample at 260 and 280 nm are 0.4857 and 0.2427, respectively. Based on the results, the sample is a. pure DNA b. pure RNA c. DNA contaminated with RNA d. DNA contaminated with protein
Enzymes that break down DNA catalyze the hydrolysis of the covalent bonds that join nucleotides together. What would happen to DNA molecules treated with these enzymes? Group of answer choices A. All bases would be separated from the deoxyribose sugars B. The purines would be separated from the deoxyribose sugars C. The phosphodiester linkages between deoxyribose sugars would be broken D. The two strands of the double helix would separate E. The pyrimidines would be separated from the deoxyribose sugars
If the nitrogen-based molecules present in the DNA of an animal are found to be 13.2% adenine molecules, what percentage of guanine would be present in the animal's DNA? a. 36.8% b. 26.4% c. 73.6% d. 13.2%
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