Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 4, Problem 5TYK
Summary Introduction

To review:

The functional groups that define the ends of a DNA (deoxyribonucleic acid) strand.

Introduction:

DNA is a double helical genetic material that is present in all the living cells. This molecule is self-replicating and replicates before the cell has to undergo cell division. Being the genetic material, DNA carries the necessary information to carry out all the functions of the cell. The structure of a DNA molecule was first given by Watson and Crick.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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