Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 4, Problem 14TYPSS
Summary Introduction

To review:

Whether the double-stranded RNA (ribonucleic acid) as the genetic material of some virus particles will form a double helix or not.

Introduction:

The genetic material is a hereditary material present inside the nucleus of all eukaryotic cells. It contains all the information required for the development and functioning of the cells. Cells can have two kinds of genetic materials, DNA (deoxyribonucleic acid) and RNA. Both DNA and RNA are a string of nucleotides linked together by phosphodiester bonds.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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