
Concept explainers
(a)
Interpretation:
The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.
Concept introduction:
Nucleotides are the construction buildings of
(b)
Interpretation:
A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.
Concept introduction:
DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the

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Chapter 4 Solutions
Biochemistry
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- Sodium fluoroacetate (FCH 2CO2Na) is a very toxic molecule that is used as rodentpoison. It is converted enzymatically to fluoroacetyl-CoA and is utilized by citratesynthase to generate (2R,3S)-fluorocitrate. The release of this product is a potentinhibitor of the next enzyme in the TCA cycle. Show the mechanism for theproduction of fluorocitrate and explain how this molecule acts as a competitiveinhibitor. Predict the effect on the concentrations of TCA intermediates.arrow_forwardIndicate for the reactions below which type of enzyme and cofactor(s) (if any) wouldbe required to catalyze each reaction shown. 1) Fru-6-P + Ery-4-P <--> GAP + Sed-7-P2) Fru-6-P + Pi <--> Fru-1,6-BP + H2O3) GTP + ADP <--> GDP + ATP4) Sed-7-P + GAP <--> Rib-5-P + Xyl-5-P5) Oxaloacetate + GTP ---> PEP + GDP + CO 26) DHAP + Ery-4-P <--> Sed-1,7-BP + H 2O7) Pyruvate + ATP + HCO3- ---> Oxaloacetate + ADP + Piarrow_forwardTPP is also utilized in transketolase reactions in the PPP. Give a mechanism for theTPP-dependent reaction between Xylulose-5-phosphate and Ribose-5-Phosphate toyield Glyceraldehyde-3-phosphate and Sedoheptulose-7-Phosphate.arrow_forward
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