Biochemistry
Biochemistry
8th Edition
ISBN: 9781464126109
Author: Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer
Publisher: W. H. Freeman
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Chapter 4, Problem 49P
Interpretation Introduction

Interpretation:

The difference in the percentage of identity of the amino acid sequence of a yeast protein and a human protein needs to be explained.

Concept introduction:

A sequence of three DNA or RNA nucleotides is called a codon. The three-nucleotide sequence or codon corresponds to a specific amino acid, and these codons are interpreted by the tRNA. The tRNA also contains the anticodon which recognizes and interprets the codons on the mRNA. The tRNA binds itscorresponding codon on the ribosome and they decode the mRNA until the whole sequence is coded.

The sequences of amino acid and that on the DNA can be very identical which what the question deals with here.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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