
Concept explainers
(a)
Interpretation:
The DNA Polymerase and RNA Polymerase from E. coli needs to be compared on the basis of the activated precursors.
Concept introduction:
DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.
RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the
(b)
Interpretation:
The DNA Polymerase and RNA Polymerase from E. coli needs to be compared on the basis of
the direction of chain elongation.
Concept introduction:
DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.
RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.
(c)
Interpretation:
The DNA Polymerase and RNA Polymerase from E.coli needs to be compared on the basis of
conservation of template.
Concept introduction:
DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.
RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.
(d)
Interpretation:
The DNA Polymerase and RNA Polymerase from E.coli needs to be compared on the basis of
need for primer.
Concept introduction:
DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.
RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.

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Chapter 4 Solutions
Biochemistry
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