The primer that can be used when a reverse transcriptase activity is supposed to be assayed where polyriboadenylate is in the template needs to be determined. Also, the radioactive nucleotide that is to be used to follow chain elongation needs to be determined. Concept introduction: Primer is a single strand of nucleic acid . It is utilized by all the living organisms for the synthesis of DNA. The primers are removed before completion of DNA replication and DNA polymerases fill the gaps in the sequence with DNA. Scientists in the laboratory can design and synthesize DNA primers with particular sequences in a single-stranded DNA molecule that bind to DNA. In order to perform the polymerase chain reaction, these DNA primres are commonly used to copy pieces. Shellac primers based on oil, latex and pigmented are three different types of primers

Question

Want to see the full answer?

Answer 1

Question

Chapter 4, Problem 22P

Interpretation Introduction

Interpretation:

The primer that can be used when a reverse transcriptase activity is supposed to be assayed where polyriboadenylate is in the template needs to be determined. Also, the radioactive nucleotide that is to be used to follow chain elongation needs to be determined.

Concept introduction:

Primer is a single strand of nucleic acid. It is utilized by all the living organisms for the synthesis of DNA. The primers are removed before completion of DNA replication and DNA polymerases fill the gaps in the sequence with DNA. Scientists in the laboratory can design and synthesize DNA primers with particular sequences in a single-stranded DNA molecule that bind to DNA.

In order to perform the polymerase chain reaction, these DNA primres are commonly used to copy pieces. Shellac primers based on oil, latex and pigmented are three different types of primers

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!