(a) Interpretation: The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined. The reason for the surprising results should be determined. Concept introduction: Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

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Answer 1

Question

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

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Answer 3

Question

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

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Answer 4

Question

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

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Answer 5

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 6

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 7

Chapter 26, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The results of graph A about the catalytic activity of the enzyme from the GD cells should be determined.

The reason for the surprising results should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 8

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 9

Interpretation Introduction

(b)

Interpretation:

Two possible explanations for the figure B should be determined.

The reason to ensure that the extracts have the same number of cells should be determined.

The purpose for including the western blot for actin should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 10

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 11

Interpretation Introduction

(c)

Interpretation:

If the amount of mRNA in both the cells is equal, then the results in figure B should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), the enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The nature of defect in GD enzyme should be determined.

The significance of the increase in activity of the enzyme from the normal cell observed in the presence of the inhibitor should be determined.

Concept introduction:

Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase), an enzyme that degrades glucocerebrosides. This disease is the most common lysosomal disease in humans. The most common symptoms of Gaucher disease is bone pain, fatigue, enlarged liver, and cognitive disabilities.

Answer 14

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Answer 17

Ch. 26 - Prob. 1P Ch. 26 - Prob. 2P Ch. 26 - Prob. 3P Ch. 26 - Prob. 4P Ch. 26 - Prob. 5P Ch. 26 - Prob. 6P Ch. 26 - Prob. 7P Ch. 26 - Prob. 8P Ch. 26 - Prob. 9P Ch. 26 - Prob. 10P

Solution Summary: The author explains that Gaucher disease is caused due to the mutation in the gene that encodes glucocerebrosidase (GCase).

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