Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Textbook Question
Chapter 20, Problem 3TYK
Gel electrophoresis is a means of separating fragments of DNA based on
- a. charge.
- b.
nucleotide sequence. - c. genetic information.
- d. size.
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Students have asked these similar questions
Which of the following best describes the process of DNA seqencing.
a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser.
b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments
c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR.
d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser
e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
What is the purpose of:
A. The concentrated salt solution?
B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent on
what factor(s)?
Select one or more:
a. G-C /A-T ratio
b. Well size of the gel
c. Size of DNA fragment
d. Volume of sample loaded
e. Negative charge of DNA
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardWhat is the purpose of the detergent in the experiment?arrow_forwardWhich of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.arrow_forward
- DNA microarrays... Select an answer and submit. For keyboard navigation, use the up/down arrow keys select an answer. a) can reveal protein-protein interactions. b b) demonstrate differential expression of genes. c) can reveal protein-RNA interactions. d. d) none of the above 2. C.arrow_forwardGel electrophoresis can be used for all of the following purposes except A) Determining the function of a gene product B Separating DNA (c) Assisting with constructing a restriction map (D) Determining the size of a DNA fragmentarrow_forwardWhich of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forward
- Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardChoose the proper order for recombinant protein purification from bacterial cells. a. lysis,pelleting by centrifugation, induction of protein expression, transformation, purification by chromatography b. induction of protein expression, transformation, pelleting by centrifugation, lysis, purification by chromatography c. transformation, induction of protein expression, pelleting by centrifugation, lysis, purification by chromatography d. purification by chromatography, lysis, pelleting by centrifugation, induction of protein expression, transformationarrow_forwardPlace the following stages of a physical mapping study in theirmost logical order:A. Clone large fragments of DNA to make a BAC library.B. Determine the DNA sequence of subclones from a cosmidlibrary.C. Subclone BAC fragments to make a cosmid library.D. Subclone cosmid fragments for DNA sequencing.arrow_forward
- With the action of detergents learned from this experiment, describe the potential harm to aquatic organisms of the practice of disposing large amounts of detergent into bodies of water.arrow_forwardWith regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy nucleotides are fluorescently labeled.b. The dideoxy nucleotides cannot be incorporated into a growingDNA strand.c. When incorporated into a DNA strand, the dideoxynucleotidesprevent further growth of the strand.d. The dideoxy sequencing method is used to determine the basesequence of DNA.e. The dideoxy sequencing method requires the use of primers.arrow_forwardThe ab initio approach finds genes by looking for a. common sequences found in most genes. b. similarity in sequence with known genes. c. mRNA with the use of in situ hybridization. d. mutant phenotypesarrow_forward
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