Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 7TYK
Summary Introduction
Introduction: Neanderthal man also known as Homo neanderthalensis is the sub species of archaic man that became extinct recently. They were named after their place of discovery, Neander valley in Germany. The genome analysis of Neanderthal man showed that they were broad, well-built with short legs, and bigger bodies compared to modern human beings.
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Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?
a.
PCR requires a copy of RNA that serves as a template
b.
Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA
c.
Annealing requires cooler temperatures than denaturation
d.
Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA
e.
PCR is a quick way of using minute quantities of DNA to create millions of copies
Choose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)?
a.
Taq Polymerase was isolated from an organism found in Yellowstone Park
b.
Restriction enzymes leave sticky ends
c.
DNA sequencing allows us to read DNA sequences
d.
Restriction enzymes cut DNA at specific sites
e.
EcoRI and HindII are commonly used polymerases
Would it be possible to use human polymerase for the PCR reaction?
a.
No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur.
b.
No, because human polymerase cannot be extracted from cells to use in a lab setting.
c.
Yes, because we are using human DNA as the template DNA.
d.
Yes, because human polymerase can add bases to a template strand without a primer.
Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardAnswer Part d,e,farrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many fragments were PRODUCED in DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forward
- Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forwardwhat of the following is more likely to speed up the loss of telomeric repeats? a.Tetreplex DNA b.Triplax DNA c.Crucieform DNA d.ZDNAarrow_forward
- The following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forwardPlace the following steps in order to outline how enzymes are involved with proofreading newly formed DNA molecules. 1. DNA polymerase does not detect damaged DNA. 2. Ligase connects the free end of the new DNA with the old DNA. 3. DNA polymerase replaces the damaged DNA with the correct nucleotide. 4. Exonuclease cuts the damaged DNA strand in order to remove the damaged section.arrow_forwardMatch the terms associated with the polymerase chain reaction with their correct descriptions. Refers to the fact that DNA molecules get longer the more of them there are in the reaction. A. В. Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other. Denaturation C. Incubate the reaction at the optimal temperature for the primers to base-pair with each other. Annealing. D. Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence. Extension. Add a chaotropic agent that destabilizes hydrogen bonding. E. Amplification. F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C). G. Happens after repeated cycles of the temperature change regimen. Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen. Н.arrow_forward
- PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardThe other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardDuring proofreading, which of the following enzymes reads the DNA? a. primase b. topoisomerase c. DNA pol d. helicasearrow_forward
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