Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 20, Problem 19TYK
Summary Introduction
Introduction: In recombinant DNA technology (rDNA technology), various tools and techniques are used to identify, amplify, and diagnose the expression of target genes. Various markers are used to locate the position of the particular gene sequences. These markers are usually radiolabeled or immune-labeled and have complementary sequence to the target genes.
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Chapter 20 Solutions
Study Guide for Campbell Biology
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardWhen bacteria are consumed by macrophages, the macrophages give off chemokines that recruit other immune cells. The chemokines serve as ligands for G-protein coupled receptors. Which of the following is NOT true of G-PCRs? a. G-PCRs require GTP to activate a G-protein b. G-PCRs bind the ligand extra-cellularly and undergo an allosteric change c. The receptor is a G-protein d. The G-protein separates into multiple parts that can activate different pathways.arrow_forward
- What are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques. Thank you!!!arrow_forwardWhen constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forward
- Genetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresis b. polymerase chain reactionc. transgenic bacteriad. DNA fingerprintingarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forwardPCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forward
- Which of the following is involved in recombinant DNA technology? Explain. MULTIPLE CHOICE. Choose only one: a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forwardWhich of the following is not required for PCR? a. dNTPs b. bacterial plasmids c. carefully designed primers d. taq or other heat- resistant polymerases e. template DNAarrow_forwardWhat is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3arrow_forward
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