Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 18, Problem 14RQ
Summary Introduction
To review:
Definition of the following terminologies:
Cohesin
Transcription factory
P bodies
CTCF
ENCODE
Introduction:
There are cellular pathways that help to transfer genetic information and encode it to the protein. These pathways are significantly ordered and regulated. Transcription is a process where deoxyribonucleic acid (DNA) transcribes into mRNA (messenger ribonucleic acid). There are various DNA and protein elements that regulate this process.
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Discuss the differences between eukaryotic and prokaryotic cells with regards to their genetic materials. Including in your discussion the structure and organisation of genetic material, as well as any implications these differences may have on cellular functions and evolution.
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9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the
MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and
14.2 kDa,
a) Figure 4: indicate where each of the measurements were taken and label as in II.6.
figure 2 above.
b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f.
values for the MW standards, plot the relative mobility versus the log of the mass
for the standards, and use the best fit straight line to determine the molecular
weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be
scaled up appropriately if your gel did not develop properly)
dye
MW
Whey
Peak 1 Peak 2
Chapter 18 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 18 - Prob. 1QCh. 18 - Prob. 2QCh. 18 - Prob. 3QCh. 18 - Prob. 4QCh. 18 - Prob. 5QCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQ
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