Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Textbook Question
Chapter 15, Problem 11TYU
These highly polymorphic molecular markers are useful in DNA fingerprinting: (a) plasmid vectors (b) cloned DNA sequences (c) palindromic DNA sequences (d) short tandem repeats (e) complementary DNAs
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In DNA technology, the term vector can refer to(A) the enzyme that cuts DNA into restrictionfragments.(B) the sticky end of a DNA fragment.(C) a SNP marker.(D) a plasmid used to transfer DNA into a living cell.
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A genetic sequence that can move from one location to an-other within a cell is known as a:(a) Bacteriocin(b) Plasmid(c) Transposon(d) Hybridoma(e) Transducible element
Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- The dideoxynucleosides ddATP, ddTTP, ddGTP, and ddCTP were important in DNA sequencing because they (a) cause premature termination of a growing DNA strand (b) are used as primers (c) cause the DNA fragments that contain them to migrate more slowly through a sequencing gel (d) are notaffected by high temperatures (e) have more energy than deoxynucleotidesarrow_forwardHuman DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) useHindIII to cut both the plasmid and the human DNA (d) a or b (e) b or carrow_forwardTissue growth factor-β (a) is a DNA probe for recombinant plasmids (b) is a product of DNA technology used in tissue engineering (c) is necessary to make cDNA (d) cannot be synthesized without a heat-resistant DNA polymerase (e) is isolated by the Southern blot techniquearrow_forward
- Which of the following tools of DNA technology is incorrectlypaired with its use?(A) electrophoresis—separation of DNA fragments(B) DNA ligase—cutting DNA, creating sticky ends of restriction fragments(C) DNA polymerase—polymerase chain reaction to amplifysections of DNA(D) reverse transcriptase—production of cDNA frommRNAarrow_forwardWhich of the following statements concerning recombinantDNA technology is false?(a) Thus far, no illnesses in laboratory workers have beentraced to genetic recombinants.(b) Production of large amounts of proteins such as insulinand human growth hormone has been made possible us-ing recombinant DNA technology.(c) Recombinant DNA technology offers specific benefitsto the scientific, medical, and general populations.(d) Mutant strains of bacteria produced by genetic re-combination are often unable to survive in the naturalenvironment.(e) Recombinant DNA technology provides a high degreeof risk to the health of the general populations.arrow_forwardARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed in the presence of ethidium bromide, supercoiled plasmid DNA binds more ethidium bromide than linear DNA. B)Tth DNA polymerase shows DNA-dependent DNA polymerase activity as well as RNA-dependent DNA polymerase activity. C)Magnesium ions stimulate polymerase activity. Therefore, it is better to use the highest concentration of magnesium in PCR amplification. D)When lambda bacteriophage infects E. coli cell lysis never occurs, and the infected bacterium can continue to grow and divide. E)The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. F)RNase H selectively hydrolyzes phosphodiester bonds of RNA molecules in RNA:DNA duplexes.arrow_forward
- 1) polymerize chain reaction is an experimental method for making multiple copies of segments of DNA from a single template without culturing cells (true or false) 2)homologous recombination is a repair mechanism that allows the transposition of DNA segments to new locations within the eukaryotic genome (true or false)arrow_forwardWhich technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardThe soil bacterium Agrobacterium tumefaciens, also regarded as 'nature's own genetic engineer', is an effective tool to create transgenic plants. This bacterial strain was crucial in the development of Golden Rice. (i) (ii) (iii) (iv) Why cannot wild type Agrobacterium tumefaciens Ti plasmids be utilized as cloning vectors? Based on your answer in 4 a) (i), explain the two (2) novel strategies introduced by scientists to enable the usage of Ti plasmids as cloning vectors. Besides A. tumefaciens, elaborate two (2) other possible methods to transfer foreign genes into plant cells. Explain briefly the steps to create a new breed of plant using this soil bacterium in the laboratory.arrow_forward
- Plants are more readily manipulated by genetic engineeringthan are animals because(A) plant genes do not contain introns.(B) more vectors are available for transferring recombinantDNA into plant cells.(C) a somatic plant cell can often give rise to a completeplant.(D) plant cells have larger nucleiarrow_forwardA paleontologist has recovered a bit of tissue from the 400-yearold preserved skin of an extinct dodo (a bird). To comparea specific region of the DNA from a sample with DNA fromliving birds, which of the following would be most useful forincreasing the amount of dodo DNA available for testing?(A) SNP analysis(B) polymerase chain reaction (PCR)(C) electroporation(D) gel electrophoresisarrow_forwardThe linking of the 5’ end of one Okazaki fragment with the 3’ end of an adjacent Okazaki fragment occurs by means of (a) DNA polymerase I (b) DNA polymerase III (c) DNA ligase (d) DNA topoisomerase (e) Primasearrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license