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Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Question
Chapter 15.2, Problem 3C
Summary Introduction
To explain: How a point mutation in a eukaryotic gene could be corrected using CRISPR/Cas9 editing.
Concept introduction: CRISPR or “clustered regularly interspaced short palindromic repeats” are short viral origin DNA repeats that are found in bacteria. Cas is CRISPR-associated endonucleases enzyme that excises the DNA. Thus, CRISPR-Cas9 recognizes the invading virus and cleaves it.
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Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- List the components that you would need in order to use the CRISPR-Cas9 system for gene editingarrow_forwardExplain how the CRISPR-Cas system defends bacteria against bacteriophages.arrow_forwardThe CRISPR-Cas9 system can be used to: a. sequence a genome in living cells b. edit a gene in living cells and organisms c. diagnose sickle-cell anemia d. identify a specific gene e. detect gene expression under different conditionsarrow_forward
- Consider the reagents required to perform CRISPR genome editing. Which molecule specifies the a new sequence that will occur at the target site if genome editing is successful? Cas9 nuclease Cas1 Cas2 tracrRNA crRNA sgRNA donor DNAarrow_forwardA scientist wants to make a random mutation in a specific gene to investigate that gene's function. Which of the following methods is she likely to have used to make this change? mutagenesis with radiation random mutagenesis CRISPR-Cas9 genome editing followed by nonhomologous end joining (NHEJ) CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template oligonucleotide-directed mutagenesisarrow_forwardDoes one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?arrow_forward
- Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzymearrow_forwardCRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however, but instead adapted from a system that already exists in nature. Describe the native biological function of CRISPR-Cas9.arrow_forwardWhich component of the CRISPR-Cas system directly recognizes thebacteriophage DNA?arrow_forward
- CRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. How can this help to create better antibiotics or to make white blood cells resistant to HIV infection? Give your examples. What is the future of CRISPR?arrow_forwardLabel the following parts of the CRISPR-Cas 9 system. C A B C D Target DNA strand PAM sequence Cas9 protein Single-guide RNAarrow_forwardWhy is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.arrow_forward
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