Biochemistry, The Molecular Basis of Life, 6th Edition
Biochemistry, The Molecular Basis of Life, 6th Edition
6th Edition
ISBN: 9780190259204
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 1, Problem 9RQ
Summary Introduction

To review:

Definition of the following biological terminologies:

(a) mRNA

(b) tRNA

(c) rRNA

(d) siRNA

(e) miRNA

Introduction:

Ribonucleic acid (RNA) is the nucleic acid found as genetic material in some viruses and bacteria. The RNA molecules are of varying types in organisms with each having a unique set of functions. Some are involved in the process of protein synthesis whereas other perform regulatory functions such as splicing and RNA silencing.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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