Biochemistry, The Molecular Basis of Life, 6th Edition
Biochemistry, The Molecular Basis of Life, 6th Edition
6th Edition
ISBN: 9780190259204
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 1, Problem 68TQ
Summary Introduction

To review:

The effect of a cholesterol-rich diet on a person.

Introduction:

A waxy substance found in body fats is known as cholesterol. It is synthesized in the cells of all animals. It is also an essential part of cell membranes of animal cells. Cholesterol plays important role in the body.

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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).  Lane 1:  buffer Lane 2 : Ladder Lane 3: Lysate Lane 4: Flowthrough Lane 5: Wash Lanes 6-8: E1, E2, E3 Lane 9: Dialyzed VRK1  Lane 10: LDH
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You have isolated a protein and determined that the native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. Describe the architecture of the polypeptide component of this enzyme.
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