You're the geneticist on a team that's uncovered a Wooly Mammoth in the ice and you want to study the function of its hemoglobin protein. First, you need to PCR amplify the gene to be able to Sanger sequence it. What reference genome do you use to design primers? O The human genome because it's the best O The bat genome because bats are cool O The elephant genome because it likely has the most sequence similarity given evolutionary distance O The E. coli genome because they're such a great model organism O The yeast genome because they're small and they're a eukaryote

You're the geneticist on a team that's uncovered a Wooly Mammoth in the ice and you want to study the function of its hemoglobin protein. First, you need to PCR amplify the gene to be able to Sanger sequence it. What reference genome do you use to design primers? O The human genome because it's the best O The bat genome because bats are cool O The elephant genome because it likely has the most sequence similarity given evolutionary distance O The E. coli genome because they're such a great model organism O The yeast genome because they're small and they're a eukaryote

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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If the primer that you design anneals to two different regions of the DNA to be sequenced, the outcome from Sanger's sequencing will be: You will obtain two sequences You won't be able to detect any C You won't have any interpretable sequencing data O You will need to use a different polymerase
Recombinant DNA technology describes a set of molecular techniques that allow us to do what with specific DNA segments? Locate Isolate Alter Investigate All of the above You're the geneticist on a team that's uncovered a Wooly Mammoth in the ice and you want to study the function of its hemoglobin protein. First, you need to PCR amplify the gene to be able to Sanger sequence it. What reference genome do you use to design primers? The human genome because it's the best The bat genome because bats are cool The elephant genome because it likely has the most sequence similarity given evolutionary distance The E. coli genome because they're such a great model organism The yeast genome because they're small and they're a eukaryote
After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you need to evaluate the results. PCR makes billions of copies of just one sequence in a sample. Since you know the sequence, you also know the length (number of nucleotides) of the region you copied. There are about 130 nucleotides in the N gene fragment. This is typically stated as 130 base pairs. How can you visualize DNA and estimate its size? Load the DNA sample into an agarose gel (similar in consistency to jello) and apply an electric current to the gel. The DNA is charged and will move through the gel. The longer the DNA fragment, the more slowly it moves. The DNA is visualized by adding a fluorescent dye to your sample that sticks to DNA. When you look at the gel under UV light, the DNA should glow. What is the charge of a DNA molecule? Based on #1, would you expect DNA to be drawn to the (+) or (-) electrode of the gel electrophoresis chamber?
Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones. O B. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers. OD. the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person.
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
The picture
If you were to sequence a human genome today, how would the sequencing differ from that done during the Human Genome Project? Choose only the best answer. You would still use Sanger sequencing. You would still assemble the sequencing reads into a genome using bioinformatics. You still need to clone the DNA fragments prior to sequencing them. All three of the other statements are true.
You want to amplify a specific region of DNA during PCR. Your primers should a Both should be an exact replica of the beginning of the specific gene you want to amplify b Both should be complementary to the beginning of the specific gene you want to amplify c One primer should be identical to the beginning of the gene sequence while the other is identical to the end of the gene sequence d One primer should be complementary to the beginning of the gene sequence while the other is complementary to the end of the gene sequence e One primer should be identical to the beginning of the gene sequence while the other is complementary to the end of the gene sequence
fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B. Size of Bearded Dragon PCR product: ______________________________ Sizes of Leopard Gecko PCR products: ______________________ yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?
Which of these is not a tool for comparing DNA sequences?
What is DNA fingerprinting? what is its application/s (legally and medically) and limitation/s? How does this technology work? i.e in genomic DNA identification? What is its molecular basis? How does molecular biology paved way for the emergence of this technology?
PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased about a billion times. Why is the DNA polymerase from the bacteria Thermus.aquaticus used in PCR? Because it uses primers specific to sequences in a disease causing gene or organism to diagnosis diseases, etc. O It is used to flank the gene to be copied. O It allows scientists to be able to determine the variable number of tandem repeats of minisatellites or microsatellites to determine an individual's DNA fingerprint. O It is present and needed because the enzyme is resistant to very high temperatures since the bacteria live in hot springs. Due to the walls of the he plant cell, which are thick; therefore it is used to degrade the walls for PCR to begin.