You have generated a new induced pluripotent stem (iPS) cell line.  How would you test for the properties of this cell line to ensure it is similar to the previous iPS cells that Yamanaka and colleagues generated?    All of the above   RNA-seq to show that the transcriptome is similar to embryonic stem cells    Generate a chimera and determine if the cell contributes to endoderm, mesoderm and ectoderm   ChIP-qPCR analysis to show active histone marks at promoters of pluripotency genes   Prolonged culture of cells to show ability to continually divide

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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You have generated a new induced pluripotent stem (iPS) cell line.  How would you test for the properties of this cell line to ensure it is similar to the previous iPS cells that Yamanaka and colleagues generated?
  
All of the above
 
RNA-seq to show that the transcriptome is similar to embryonic stem cells 
 
Generate a chimera and determine if the cell contributes to endoderm, mesoderm and ectoderm
 
ChIP-qPCR analysis to show active histone marks at promoters of pluripotency genes
 
Prolonged culture of cells to show ability to continually divide 
 
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