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controlling the cell cycle. Earlier researchers had found that extra om frog eggs (Xenopus) contained all the necessary proteins and m quired for DNA replication. This included proteins that regulated tl omoting factor (MPF). At the time of this study, cyclin B was show tivity and the research group wanted to test using Xenopus egg ex: say. In Figure 1 (a) MPF activity was tested for its ability to phosph stone (H1) in sperm chromatin over a certain period of time. Addit clin B concentration in the extract was measured.

controlling the cell cycle. Earlier researchers had found that extra om frog eggs (Xenopus) contained all the necessary proteins and m quired for DNA replication. This included proteins that regulated tl omoting factor (MPF). At the time of this study, cyclin B was show tivity and the research group wanted to test using Xenopus egg ex: say. In Figure 1 (a) MPF activity was tested for its ability to phosph stone (H1) in sperm chromatin over a certain period of time. Addit clin B concentration in the extract was measured.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In Figure 1d, cyclin B MRNA which produced a cyclin B protein that could not be degraded was added to the RNase -treated extract (fig 1b) and the activity was measured. What happened to the mitosis cycle? When comparing Fig1d with Fig la (or Fig 1c), what would this indicate regarding cyclin B in terms of mitosis cycling?
Transcribed Image Text:In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In Figure 1d, cyclin B MRNA which produced a cyclin B protein that could not be degraded was added to the RNase -treated extract (fig 1b) and the activity was measured. What happened to the mitosis cycle? When comparing Fig1d with Fig la (or Fig 1c), what would this indicate regarding cyclin B in terms of mitosis cycling?
Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
Transcribed Image Text:Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
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