You are studying lung cancer and use a microarray to compare gene expression between a lung cancer tumor and healthy adjacent lung tissue. If you label vour cancer cDNA with red fiuorescence and your normal tissue with green, what color would you expect tumor suppressors to be: © a. Green U D. Red Oc.No color ) d. Either Red or Green © e Yellow
1- You are studying lung cancer and use a microarray to compare gene expression between a lung cancer tumor and healthy adjacent lung tissue. If you label vour cancer cDNA
with red fiuorescence and your normal tissue with green, what color would you expect tumor suppressors to be:
© a. Green
U D. Red
Oc.No color
) d. Either Red or Green
© e Yellow
2- You want to study histone modifications using Next Generation sequencing. What technique would allow you to do this?
© a, Sanger sequencing
© b. Fluorescence in situ hybl
© c. RNA-Seq
Old. ChIP-sed
© e. Poymerase Chain Reaction
3-Gertrudae mcfuzzi is a rare (and ficticious) species of bird with colorful tail feathers. All Gertrude mcfuzzi develop 1 tail feather when they are born, and can grow additional
tall teathers during matunty depending on the alleles they inherit at a number of additive loci (T, S, and G) which form the geise/-complex. Each positive (+) allele adds one lal
feather and each negative - allele produces no additional feathers, based on the amount of geisel-complex hormone produced
If a male T+T+S+S-G+G. mated with a female T+T.S.S.G.G
snowmel
possible phenotvpes can be found in the offspring?
1
2
3
4
5
6
4-In Burkitt lymphoma, there is increased expression of the MYC gene. Which of the following best explains this situation?
O a.
A chromosome deletion has removed a tumor-suppressor gene
© b. The MYC gene has been amplified
U c. A chromosome deletion was removed an oncogene
© d. A chromosome duplication that involves a segment with an oncogene
• e. A translocation has brought the myc
gene next to a different regulatory region
5-Which of the following is required for repairing the phosphodiester backbone of DNA during molecular cloning?
© restriction enzymes
© reverse transcriptase
© CDNA
DNA ligase
deaminase
7-The first draft of the human genome. published in 2003. used which technique(s) to complete it? (Choose all that apply)
7 a. Shotgun sequencing
O b. Sanger sequencing
O c chiP-seg
O d.RNA-seq
We. Mapped based sequencing
Of. Next-Generation sequencing
g. Pacbio sequencing
8-How much of the human genome codes for proteins?
©a approximately 50 %
© b. Approximately 74%
O c. Approximately 24%
© d. Less than 2%
© e. More than 82%
10- Huntington's disease is an example of a disease caused by what type of mutation?
© a missense mutation
© b. Expanding
© C. Frameshift mutation
Nonsense mutation
©e. Gain-of-function
11-Rank the following regions or types of mutations in order of lowest to highest mutation rates generally observed across the genome
© mutations in introns <
mutations in pseudogenes < non-synonymous substitutions
© mutations in pseudogenes < mutations in introns < non-synonymous substitutions
non-synonymous substitutions < mutations in pseudogenes < mutations in introns
© mutations in pseudogenes < non-synonymous substitutions < mutations in introns
© non-synonymous substitutions < mutations in introns « mutations in pseudogenes
© mutations in introns < non-synonymous substitutions < mutations in pseudogenes
12. During agarose gel electrophoresis. DNA will be attracted to the negative alon-ad
O Tue
© false
13. Below are the sequences of 5 fragments of DNA
5' -GTCTGAACCTI-3
5-AGACCCTTAAA-3'
5' -CIGATCTAGCTAGCT-3
5 -ACCTTCGATCTGAT-3
5' -TTAAATCTGTCTG-3'
Order these fragments to form a contig
a.c.db.e.e
0b.a.e.0bd
c.b.dcee
Od.b.e.a,d,c
Oe.a.c.0,0.0
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