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4. To understand the function of the Bcl11a protein, scientists attempted to knockdown its expression by transfecting human cells in culture with siRNA targeting the Bcl11a gene. A. Briefly describe the first step of the pathway initiated by siRNA entering the cytoplasm. Following transfection of cells with two different siRNA sequences complementary to the Bcl11a gene (siRNA 1 and siRNA 2), the level of Bcl11a protein expression was measured by western blot. The researchers also included a positive control to measure actin, a housekeeping gene. In a western blot experiment*, how do scientists B. specifically detect a protein of interest on a nitrocellulose membrane? Control siRNA 1 siRNA 2 C. Which siRNA sequence was more effective at targeting Bcl11a expression? Briefly explain your answer. BCL11 Actin

4. To understand the function of the Bcl11a protein, scientists attempted to knockdown its expression by transfecting human cells in culture with siRNA targeting the Bcl11a gene. A. Briefly describe the first step of the pathway initiated by siRNA entering the cytoplasm. Following transfection of cells with two different siRNA sequences complementary to the Bcl11a gene (siRNA 1 and siRNA 2), the level of Bcl11a protein expression was measured by western blot. The researchers also included a positive control to measure actin, a housekeeping gene. In a western blot experiment*, how do scientists B. specifically detect a protein of interest on a nitrocellulose membrane? Control siRNA 1 siRNA 2 C. Which siRNA sequence was more effective at targeting Bcl11a expression? Briefly explain your answer. BCL11 Actin

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4. To understand the function of the Bcl11a protein, scientists attempted to knockdown its expression by transfecting human cells in culture with siRNA targeting the Bcl11a gene. A. Briefly describe the first step of the pathway initiated by siRNA entering the cytoplasm. Following transfection of cells with two different siRNA sequences complementary to the Bcl11a gene (siRNA 1 and siRNA 2), the level of Bcl11a protein expression was measured by western blot. The researchers also included a positive control to measure actin, a housekeeping gene. In a western blot experiment*, how do scientists B. specifically detect a protein of interest on a nitrocellulose membrane? Control siRNA 1 C. Which siRNA sequence was more effective at targeting Bcl11a expression? Briefly explain your answer. siRNA 2 BCL11A Actin
Transcribed Image Text:4. To understand the function of the Bcl11a protein, scientists attempted to knockdown its expression by transfecting human cells in culture with siRNA targeting the Bcl11a gene. A. Briefly describe the first step of the pathway initiated by siRNA entering the cytoplasm. Following transfection of cells with two different siRNA sequences complementary to the Bcl11a gene (siRNA 1 and siRNA 2), the level of Bcl11a protein expression was measured by western blot. The researchers also included a positive control to measure actin, a housekeeping gene. In a western blot experiment*, how do scientists B. specifically detect a protein of interest on a nitrocellulose membrane? Control siRNA 1 C. Which siRNA sequence was more effective at targeting Bcl11a expression? Briefly explain your answer. siRNA 2 BCL11A Actin
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